Thermo Scientific Pierce Protein Assay Technical Handbook Version 2
Thermo Scientific Pierce Protein Assay Technical Handbook Version 2
Thermo Scientific Pierce Protein Assay Technical Handbook Version 2
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Coomassie Dye-based <strong>Protein</strong> <strong>Assay</strong>s<br />
Coomassie (Bradford) <strong>Protein</strong> <strong>Assay</strong><br />
The Bradford method workhorse … ready-to-use, allowing total<br />
protein determination in seconds!<br />
0.03mL sample + 1.5mL<br />
Coomassie Reagent<br />
Read at 595nm<br />
This ready-to-use formulation more closely resembles in performance,<br />
the reagent published by Bradford. 1 It demonstrates the<br />
typical assay characteristics known for Coomassie dye-based<br />
formulations. 2<br />
Highlights:<br />
• Ready-to-use dye-binding reagent formulation<br />
• Fast (almost immediate) color development; read at 595nm<br />
• Compatible with reducing substances and chelating agents<br />
• Refrigerated reagent is stable for 12 months<br />
• Determine protein concentration from 100 to 1,500µg/mL<br />
• Micro method for the range of 1 to 25µg/mL<br />
• Convenient microplate or cuvette format<br />
Net Absrobance (595nm)<br />
1.75<br />
1.50<br />
1.25<br />
1.00<br />
0.75<br />
0.50<br />
0.25<br />
Mix well<br />
Spectrophotometer<br />
<strong>Thermo</strong> <strong>Scientific</strong> <strong>Pierce</strong> Coomassie (Bradford) <strong>Protein</strong> <strong>Assay</strong> protocol.<br />
Ordering Information<br />
Product Description Pkg. Size<br />
23200 Coomassie (Bradford) <strong>Protein</strong> <strong>Assay</strong> Kit<br />
(Ready-to-use Coomassie Blue G-250<br />
based reagent)<br />
Sufficient reagents to perform 630 standard tube<br />
assays or 3,800 microplate assays.<br />
Includes: Coomassie <strong>Protein</strong> <strong>Assay</strong> Reagent<br />
Albumin Standard Ampules (2mg/mL)<br />
Kit<br />
950mL<br />
10 x 1mL<br />
References<br />
1. Bradford, M. (1976). Anal. Biochem. 72, 248-254.<br />
2. VanKley, H. and Hale, S.M. (1977). Anal. Biochem. 81, 485-487.<br />
Messenger, M.M., et al. (2002). J. Biol Chem. 277, 23054-23064.<br />
0.00<br />
0<br />
500<br />
1,000<br />
1,500<br />
<strong>Protein</strong> Concentration (µg/mL)<br />
2,000<br />
<strong>Thermo</strong> <strong>Scientific</strong> <strong>Pierce</strong> Coomassie (Bradford) <strong>Protein</strong> <strong>Assay</strong><br />
Reagent: typical color response curve for BSA.<br />
24<br />
For more information, or to download product instructions, visit www.thermoscientific.com/pierce