Thermoelectric Properties of Fe0.2Co3.8Sb12-xTex ... - Physics
Thermoelectric Properties of Fe0.2Co3.8Sb12-xTex ... - Physics
Thermoelectric Properties of Fe0.2Co3.8Sb12-xTex ... - Physics
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
Unzipping Force Analysis to determine binding specificity <strong>of</strong><br />
RNA Polymerase to T7A1 promoter sequence<br />
Sudeesh K †,1 , Subho Ghosh* ,1 , Dipankar Chatterji*, A K Sood †<br />
† Department <strong>of</strong> <strong>Physics</strong>, Indian Institute <strong>of</strong> Science<br />
* Molecular Biophysics unit, Indian Institute <strong>of</strong> Science<br />
Abstract:- Transciption is one <strong>of</strong> the important steps in the central dogma <strong>of</strong> molecular biology. Using<br />
RNA Polymerase <strong>of</strong> Escherichia coli and T7A1 promoter from T7 phage as the model system, we<br />
probe the sequence dependence <strong>of</strong> the unzipping force <strong>of</strong> transcription at the single molecule level. We<br />
unzip wild type and various mutated sequences <strong>of</strong> DNA with RNA-polymerase attached to each <strong>of</strong><br />
them and determine the change in the binding force at the beginning <strong>of</strong> the unzipping <strong>of</strong> the<br />
transcription bubble. We thus determine the role <strong>of</strong> each individual base pair <strong>of</strong> the consensus<br />
promoter sequence affecting the binding <strong>of</strong> RNA-Polymerase to the promoter.<br />
1) S.K and S.G contributed equally for the work
Change language