Eliminating manual steps in a high throughput ... - PerkinElmer

Eliminating manual steps in a high throughput ... - PerkinElmer Eliminating manual steps in a high throughput ... - PerkinElmer

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Process Flow Genotyping 2010: Setup Define Geno- Assay Tail DNA Assay Dev. Breeding typing Task Design Prep SOP / DB Manual Pipetting Fragment Analysis Export to Database Quality Control Automated Pipetting Fragment Export to Quality Analysis Database Control Manual tasks Automated procedures, no manual interferering

…everything done!! ‣Overview about PCRs per animal facility plate ‣ Creates: - Mastermix recipies (Setup Validator) - Pipetting instructions (Hamilton robotics) - Layout-file for LabChip (Sample name, position, expected peak ) ‣ Automated data entry in MouseHouse database Tails in 96well plate Layout file Typing results Typing requests Pipetting instructions Result file Mouse House Pipetteted, cycled plates

Process Flow Genotyp<strong>in</strong>g 2010:<br />

Setup<br />

Def<strong>in</strong>e Geno-<br />

Assay<br />

Tail DNA<br />

Assay Dev.<br />

Breed<strong>in</strong>g typ<strong>in</strong>g Task Design Prep<br />

SOP / DB<br />

Manual<br />

Pipett<strong>in</strong>g<br />

Fragment<br />

Analysis<br />

Export to<br />

Database<br />

Quality<br />

Control<br />

Automated<br />

Pipett<strong>in</strong>g<br />

Fragment Export to Quality<br />

Analysis Database Control<br />

Manual tasks<br />

Automated procedures,<br />

no <strong>manual</strong> <strong>in</strong>terferer<strong>in</strong>g

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