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Fully Automated Sample Processing and HTP ... - PerkinElmer

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Value of Protease Deficient Hosts<br />

Problem: Yeast toolbox ineffective resulting in proteolytic degradation, very high<br />

heterogeneous glycosylation<br />

“Stacked” protease deletion strain with very high titer (>1 g/L in 96 well), superior<br />

product quality, no degradation or heterogeneity, soluble <strong>and</strong> active protein<br />

Protease Knockout<br />

Null 1 2 1+2 STD Spike<br />

0.5mg/mL<br />

Host Strain Choice Controls<br />

Expression Level <strong>and</strong> Quality<br />

Full length<br />

clipped<br />

Discovery ● Development ● Production<br />

9

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