Fully Automated Sample Processing and HTP ... - PerkinElmer
Fully Automated Sample Processing and HTP ... - PerkinElmer Fully Automated Sample Processing and HTP ... - PerkinElmer
Value of Secretion Leader Screening 96 well HTP growth; CGE analysis 10 unique leaders + scFv (27kDa) Soluble Leaders, 3 lanes each null A B C D Cyto E F G H I J Insoluble Varying levels of processing, solubility and target yield with different secretion leaders Discovery ● Development ● Production 8
Value of Protease Deficient Hosts Problem: Yeast toolbox ineffective resulting in proteolytic degradation, very high heterogeneous glycosylation “Stacked” protease deletion strain with very high titer (>1 g/L in 96 well), superior product quality, no degradation or heterogeneity, soluble and active protein Protease Knockout Null 1 2 1+2 STD Spike 0.5mg/mL Host Strain Choice Controls Expression Level and Quality Full length clipped Discovery ● Development ● Production 9
- Page 1 and 2: Fully Automated Sample Processing a
- Page 3 and 4: Who we are: • Located in San Dieg
- Page 5 and 6: Pfēnex HTP Strain Development >85%
- Page 7: Periplasmic Targeting of Recombinan
- Page 11 and 12: Process analytical: 1 st and 2 nd t
- Page 13 and 14: Expression analysis: Sample Prepara
- Page 15 and 16: Robotically Enabled Sample Preparat
- Page 17 and 18: Structure of the influenza hemagglu
- Page 19 and 20: Strains Selected for Fermentation R
- Page 21 and 22: 4-mL Fermentation PhyTip-IMAC Solub
- Page 23 and 24: Downstream Processing Robotic Batch
- Page 25 and 26: Summary Extensive genomics capabili
- Page 27: Acknowledgements: Pfenex Lauren Alc
Value of Secretion Leader Screening<br />
96 well <strong>HTP</strong> growth; CGE analysis<br />
10 unique leaders + scFv (27kDa)<br />
Soluble<br />
Leaders,<br />
3 lanes each<br />
null A B C D Cyto E F G H I J<br />
Insoluble<br />
Varying levels of processing, solubility <strong>and</strong> target yield with different secretion leaders<br />
Discovery ● Development ● Production<br />
8
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