Fully Automated Sample Processing and HTP ... - PerkinElmer
Fully Automated Sample Processing and HTP ... - PerkinElmer Fully Automated Sample Processing and HTP ... - PerkinElmer
Pfēnex Platform: The New Standard for Production Strain Engineering and Protein Production DNA constructs UPSTREAM DOWNSTREAM MIDSTREAM strain selection fermentation primary recovery purification characterization and analysis data analysis/management & bioinformatics process analytical 2 plasmids 4+ promoters 3 ribosome binding sites 25 secretion leaders 30 chaperone/disulfide bond isomerase overexpression plasmids 100+ protease clean deletion mutants, multiple deletions Thousands of unique combinations enable optimal strain discovery for biopharmaceutical protein production Discovery ● Development ● Production 6
Periplasmic Targeting of Recombinant Proteins Outer membrane Periplasm Inner membrane DNA Promoter +1 mRNA Cytoplasm Folding Modulators Disulfide Bond Formation Signal peptide Terminator Gene of Interest Shine-Dalgarno Oxido–reductases DsbA and DsbB Disulfide isomerases DsbD, DsbC, and DsbG Sec Recombinant Protein Signal peptidase Inactive Active Protein Extracellular space Simplified recovery Discovery ● Development ● Production
- Page 1 and 2: Fully Automated Sample Processing a
- Page 3 and 4: Who we are: • Located in San Dieg
- Page 5: Pfēnex HTP Strain Development >85%
- Page 9 and 10: Value of Protease Deficient Hosts P
- Page 11 and 12: Process analytical: 1 st and 2 nd t
- Page 13 and 14: Expression analysis: Sample Prepara
- Page 15 and 16: Robotically Enabled Sample Preparat
- Page 17 and 18: Structure of the influenza hemagglu
- Page 19 and 20: Strains Selected for Fermentation R
- Page 21 and 22: 4-mL Fermentation PhyTip-IMAC Solub
- Page 23 and 24: Downstream Processing Robotic Batch
- Page 25 and 26: Summary Extensive genomics capabili
- Page 27: Acknowledgements: Pfenex Lauren Alc
Pfēnex Platform:<br />
The New St<strong>and</strong>ard for Production Strain Engineering <strong>and</strong> Protein Production<br />
DNA constructs<br />
UPSTREAM<br />
DOWNSTREAM MIDSTREAM<br />
strain selection<br />
fermentation<br />
primary recovery<br />
purification<br />
characterization <strong>and</strong><br />
analysis<br />
data analysis/management & bioinformatics<br />
process analytical<br />
2<br />
plasmids<br />
4+ promoters<br />
3 ribosome binding sites<br />
25 secretion leaders<br />
30 chaperone/disulfide bond<br />
isomerase overexpression plasmids<br />
100+ protease clean deletion mutants, multiple deletions<br />
Thous<strong>and</strong>s of unique combinations enable optimal strain<br />
discovery for biopharmaceutical protein production<br />
Discovery ● Development ● Production<br />
6
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