Fully Automated Sample Processing and HTP ... - PerkinElmer

Pfēnex HTP Strain Development
>85% Success Rate for Proteins that Previously Failed in Other System
Examples of Stalled Development:
Protein Type Alternative Host Pfēnex Results
Fab Yeast: quality issues, low yield 10-20X yield improvement
high quality at 1L scale
Microbial outer membrane protein E. coli: no expression Soluble active expression g/L;
1.0L scale
Growth Factor
Therapeutic Enzyme
Human Cytokine
Yeast: low yield, degradation,
glycosylation
E. coli: undesirable isoforms,
quality issues
E. coli- inclusion bodies; no
soluble expression
20X yield improvement at HTP
scale, high quality, active
10X yield improvement; no
isoform issues
Soluble active expression;
elimination of refold step
Multimeric Antibody Derivative CHO- low expression(