Fully Automated Sample Processing and HTP ... - PerkinElmer
Fully Automated Sample Processing and HTP ... - PerkinElmer Fully Automated Sample Processing and HTP ... - PerkinElmer
Pfēnex Expression Technology • P. fluorescens is a nonpathogenic, Gram-negative, obligate aerobic, bacterium • Genomic, “systems biology” approach to host strain construction enables rapid and reliable strain development • Combine expression plasmids for multiple expression strategies (promoter, ribosome binding site & secretion signal) with multiple host strains • Develop effective high throughput growth and assay methods • Rapid development of production strains yielding high titers of quality protein • Altered paradigm: Discard linear, iterative approach, adopt parallel, high throughput method for microbial strain development Discovery ● Development ● Production 4
Pfēnex HTP Strain Development >85% Success Rate for Proteins that Previously Failed in Other System Examples of Stalled Development: Protein Type Alternative Host Pfēnex Results Fab Yeast: quality issues, low yield 10-20X yield improvement high quality at 1L scale Microbial outer membrane protein E. coli: no expression Soluble active expression g/L; 1.0L scale Growth Factor Therapeutic Enzyme Human Cytokine Yeast: low yield, degradation, glycosylation E. coli: undesirable isoforms, quality issues E. coli- inclusion bodies; no soluble expression 20X yield improvement at HTP scale, high quality, active 10X yield improvement; no isoform issues Soluble active expression; elimination of refold step Multimeric Antibody Derivative CHO- low expression(
- Page 1 and 2: Fully Automated Sample Processing a
- Page 3: Who we are: • Located in San Dieg
- Page 7 and 8: Periplasmic Targeting of Recombinan
- Page 9 and 10: Value of Protease Deficient Hosts P
- Page 11 and 12: Process analytical: 1 st and 2 nd t
- Page 13 and 14: Expression analysis: Sample Prepara
- Page 15 and 16: Robotically Enabled Sample Preparat
- Page 17 and 18: Structure of the influenza hemagglu
- Page 19 and 20: Strains Selected for Fermentation R
- Page 21 and 22: 4-mL Fermentation PhyTip-IMAC Solub
- Page 23 and 24: Downstream Processing Robotic Batch
- Page 25 and 26: Summary Extensive genomics capabili
- Page 27: Acknowledgements: Pfenex Lauren Alc
Pfēnex Expression Technology<br />
• P. fluorescens is a nonpathogenic, Gram-negative, obligate aerobic,<br />
bacterium<br />
• Genomic, “systems biology” approach to host strain construction enables<br />
rapid <strong>and</strong> reliable strain development<br />
• Combine expression plasmids for multiple expression strategies (promoter,<br />
ribosome binding site & secretion signal) with multiple host strains<br />
• Develop effective high throughput growth <strong>and</strong> assay methods<br />
• Rapid development of production strains yielding high titers of quality<br />
protein<br />
• Altered paradigm: Discard linear, iterative approach, adopt parallel, high<br />
throughput method for microbial strain development<br />
Discovery ● Development ● Production<br />
4
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