Fully Automated Sample Processing and HTP ... - PerkinElmer
Fully Automated Sample Processing and HTP ... - PerkinElmer Fully Automated Sample Processing and HTP ... - PerkinElmer
IMAC Parameter Screen: SDS-CGE gel-like images pH 7 pH 9 0 mM 250 mM 500 mM NaCl 0 mM 250 mM 500 mM NaCl pH 8 0 mM 250 mM 500 mM NaCl Best conditions transferred Discovery ● Development ● Production Pfenex Confidential, Not US government
Summary Extensive genomics capability for rapid, precise strain and process improvement, and for support of product characterization Multi-dimensional approach to gene expression, protein recovery Continued effort in automation for increased efficiencies and robust analytical processes automation of sample preparation alleviates key bottleneck Advantaged production of soluble and active target proteins, simplifying downstream processing and enhancing yields Caliper’s Labchip technology is fully integrated across the Pfenex platform Discovery ● Development ● Production 25
- Page 1 and 2: Fully Automated Sample Processing a
- Page 3 and 4: Who we are: • Located in San Dieg
- Page 5 and 6: Pfēnex HTP Strain Development >85%
- Page 7 and 8: Periplasmic Targeting of Recombinan
- Page 9 and 10: Value of Protease Deficient Hosts P
- Page 11 and 12: Process analytical: 1 st and 2 nd t
- Page 13 and 14: Expression analysis: Sample Prepara
- Page 15 and 16: Robotically Enabled Sample Preparat
- Page 17 and 18: Structure of the influenza hemagglu
- Page 19 and 20: Strains Selected for Fermentation R
- Page 21 and 22: 4-mL Fermentation PhyTip-IMAC Solub
- Page 23: Downstream Processing Robotic Batch
- Page 27: Acknowledgements: Pfenex Lauren Alc
Summary<br />
Extensive genomics capability for rapid, precise strain <strong>and</strong> process<br />
improvement, <strong>and</strong> for support of product characterization<br />
Multi-dimensional approach to gene expression, protein recovery<br />
Continued effort in automation for increased efficiencies <strong>and</strong> robust<br />
analytical processes automation of sample preparation alleviates<br />
key bottleneck<br />
Advantaged production of soluble <strong>and</strong> active target proteins, simplifying<br />
downstream processing <strong>and</strong> enhancing yields<br />
Caliper’s Labchip technology is fully integrated across the Pfenex<br />
platform<br />
Discovery ● Development ● Production<br />
25
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