Fully Automated Sample Processing and HTP ... - PerkinElmer

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1L Fermentation - PhyTip-IMAC Soluble Fractions SDS-CGE Images Mw 0 8 16 24 0 8 16 24 0 8 16 24 0 8 16 24 0 8 16 24 hr post-induction SUMO-HA TAG-HA Best strain and condition transferred Discovery ● Development ● Production Pfenex Confidential, Not US government
Downstream Processing Robotic Batch Screen PhyTip and/or filter plates containing resins apply target, filter, wash, elute with different parameters Scouting and Optimization HTP Analysis Batch screen- robotically-enabled microtiter plate or PhyTip format; test resin for binding capacity/selectivity using varying conditions Scouting- small columns to test screen leads; comparative test gradients; variable scouting; dynamic binding Optimization- fine tune parameters using scaled-down larger column (pH, protein loading, flow velocity) Scale-up Bench-scale Chromatography Pilot-scale Chromatography Discovery ● Development ● Production
Page 1 and 2: Fully Automated Sample Processing a
Page 3 and 4: Who we are: • Located in San Dieg
Page 5 and 6: Pfēnex HTP Strain Development >85%
Page 7 and 8: Periplasmic Targeting of Recombinan
Page 9 and 10: Value of Protease Deficient Hosts P
Page 11 and 12: Process analytical: 1 st and 2 nd t
Page 13 and 14: Expression analysis: Sample Prepara
Page 15 and 16: Robotically Enabled Sample Preparat
Page 17 and 18: Structure of the influenza hemagglu
Page 19 and 20: Strains Selected for Fermentation R
Page 21: 4-mL Fermentation PhyTip-IMAC Solub
Page 25 and 26: Summary Extensive genomics capabili
Page 27: Acknowledgements: Pfenex Lauren Alc

Fully Automated Sample Processing and HTP ... - PerkinElmer

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