Fully Automated Sample Processing and HTP ... - PerkinElmer

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Fermentation Work Flow Fermentation scouting • 8 strains • 17 variable induction conditions each Fermentation confirmation • 3strains • 1-3 variable induction conditions each 24-unit 4 mL 8-unit 1.0 L From 1 L fermentations: • Multiple time-point samples taken for expression analysis • Cultures harvested to generate lysates/extracts Discovery ● Development ● Production
4-mL Fermentation PhyTip-IMAC Soluble Fractions Top soluble strains Induction condition Strain #1 TAG-HA Strain #2 TAG-HA SDS-CGE Images Discovery ● Development ● Production Pfenex Confidential, Not US government
Page 1 and 2: Fully Automated Sample Processing a
Page 3 and 4: Who we are: • Located in San Dieg
Page 5 and 6: Pfēnex HTP Strain Development >85%
Page 7 and 8: Periplasmic Targeting of Recombinan
Page 9 and 10: Value of Protease Deficient Hosts P
Page 11 and 12: Process analytical: 1 st and 2 nd t
Page 13 and 14: Expression analysis: Sample Prepara
Page 15 and 16: Robotically Enabled Sample Preparat
Page 17 and 18: Structure of the influenza hemagglu
Page 19: Strains Selected for Fermentation R
Page 23 and 24: Downstream Processing Robotic Batch
Page 25 and 26: Summary Extensive genomics capabili
Page 27: Acknowledgements: Pfenex Lauren Alc

Fully Automated Sample Processing and HTP ... - PerkinElmer

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