Fully Automated Sample Processing and HTP ... - PerkinElmer
Fully Automated Sample Processing and HTP ... - PerkinElmer Fully Automated Sample Processing and HTP ... - PerkinElmer
Fermentation Work Flow Fermentation scouting • 8 strains • 17 variable induction conditions each Fermentation confirmation • 3strains • 1-3 variable induction conditions each 24-unit 4 mL 8-unit 1.0 L From 1 L fermentations: • Multiple time-point samples taken for expression analysis • Cultures harvested to generate lysates/extracts Discovery ● Development ● Production
4-mL Fermentation PhyTip-IMAC Soluble Fractions Top soluble strains Induction condition Strain #1 TAG-HA Strain #2 TAG-HA SDS-CGE Images Discovery ● Development ● Production Pfenex Confidential, Not US government
- Page 1 and 2: Fully Automated Sample Processing a
- Page 3 and 4: Who we are: • Located in San Dieg
- Page 5 and 6: Pfēnex HTP Strain Development >85%
- Page 7 and 8: Periplasmic Targeting of Recombinan
- Page 9 and 10: Value of Protease Deficient Hosts P
- Page 11 and 12: Process analytical: 1 st and 2 nd t
- Page 13 and 14: Expression analysis: Sample Prepara
- Page 15 and 16: Robotically Enabled Sample Preparat
- Page 17 and 18: Structure of the influenza hemagglu
- Page 19: Strains Selected for Fermentation R
- Page 23 and 24: Downstream Processing Robotic Batch
- Page 25 and 26: Summary Extensive genomics capabili
- Page 27: Acknowledgements: Pfenex Lauren Alc
Fermentation Work Flow<br />
Fermentation scouting<br />
• 8 strains<br />
• 17 variable induction conditions each<br />
Fermentation confirmation<br />
• 3strains<br />
• 1-3 variable induction conditions each<br />
24-unit 4 mL<br />
8-unit 1.0 L<br />
From 1 L fermentations:<br />
• Multiple time-point samples taken for expression analysis<br />
• Cultures harvested to generate lysates/extracts<br />
Discovery ● Development ● Production
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