Fully Automated Sample Processing and HTP ... - PerkinElmer

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HTP Growth and Expression Analysis Workflow seed pXXX + Multiple Expression Plasmids Pfēnex Multiple Hosts, Multiple Phenotypes 320 unique expression strains constructed Electroporate (96 well) and grow transformants on selective medium Prepare seed plate Grow triplicates for each recombinant strain HTP Expression SDS-CGE Titer Analyze soluble / insoluble cellular fractions CHOOSE BEST STRAINS Normalize to similar density, Sonicate to lyse cells 30-50 OD 600 in 96 well plates Discovery ● Development ● Production
Strains Selected for Fermentation Relative yield by SDS-CGE Top strains First data set complete 4 hours after harvest! IMAC SDS-CGE analysis of top strains null Tag-HA Selected samples of soluble fractions were purified by PhyTips with IMAC resin Discovery ● Development ● Production Pfenex Confidential, Not US government
Page 1 and 2: Fully Automated Sample Processing a
Page 3 and 4: Who we are: • Located in San Dieg
Page 5 and 6: Pfēnex HTP Strain Development >85%
Page 7 and 8: Periplasmic Targeting of Recombinan
Page 9 and 10: Value of Protease Deficient Hosts P
Page 11 and 12: Process analytical: 1 st and 2 nd t
Page 13 and 14: Expression analysis: Sample Prepara
Page 15 and 16: Robotically Enabled Sample Preparat
Page 17: Structure of the influenza hemagglu
Page 21 and 22: 4-mL Fermentation PhyTip-IMAC Solub
Page 23 and 24: Downstream Processing Robotic Batch
Page 25 and 26: Summary Extensive genomics capabili
Page 27: Acknowledgements: Pfenex Lauren Alc

Fully Automated Sample Processing and HTP ... - PerkinElmer

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