Fully Automated Sample Processing and HTP ... - PerkinElmer
Fully Automated Sample Processing and HTP ... - PerkinElmer
Fully Automated Sample Processing and HTP ... - PerkinElmer
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<strong>HTP</strong> Growth <strong>and</strong> Expression Analysis Workflow<br />
seed<br />
pXXX<br />
+<br />
Multiple Expression<br />
Plasmids<br />
Pfēnex<br />
Multiple Hosts,<br />
Multiple<br />
Phenotypes<br />
320 unique expression<br />
strains constructed<br />
Electroporate (96<br />
well) <strong>and</strong> grow<br />
transformants on<br />
selective medium<br />
Prepare seed<br />
plate<br />
Grow triplicates<br />
for each<br />
recombinant<br />
strain<br />
<strong>HTP</strong> Expression<br />
SDS-CGE<br />
Titer<br />
Analyze soluble / insoluble cellular fractions<br />
CHOOSE BEST STRAINS<br />
Normalize to<br />
similar density,<br />
Sonicate to lyse<br />
cells<br />
30-50 OD 600 in 96 well plates<br />
Discovery ● Development ● Production
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