Fully Automated Sample Processing and HTP ... - PerkinElmer
Fully Automated Sample Processing and HTP ... - PerkinElmer Fully Automated Sample Processing and HTP ... - PerkinElmer
HTP Microchip SDS-CGE Labchip ® GXII Protein chip Schematic layout of the microchannels of protein chip. The red channels are filled with sieving matrix. LC90 Features of the Labchip GXII: • Capable of interfacing with plate robotics • Increased chip stability • On-line chip priming • Dry focusing • Chip RFID reader keeps user informed of onchip reagent and chip lifetime status • CFR21 Part 11 compliance • Great improvements in software Discovery ● Development ● Production 12
Expression analysis: Sample Preparation Bottleneck challenge: to accurately analyze target in complex mixture of solids, protein, DNA, etc. in timely fashion samples: whole cells HTP strain selection 1000s of samples Fermentation Optimization sample handling and cell lysis separation of soluble and insoluble fractions analysis 100s of samples Goal “Old” process steps semi-automated, manual labels, labor intensive centrifugation in multiple rounds; manual handling Improve throughput and efficiencies through automation while maintaining high-quality analytical support Discovery ● Development ● Production
- Page 1 and 2: Fully Automated Sample Processing a
- Page 3 and 4: Who we are: • Located in San Dieg
- Page 5 and 6: Pfēnex HTP Strain Development >85%
- Page 7 and 8: Periplasmic Targeting of Recombinan
- Page 9 and 10: Value of Protease Deficient Hosts P
- Page 11: Process analytical: 1 st and 2 nd t
- Page 15 and 16: Robotically Enabled Sample Preparat
- Page 17 and 18: Structure of the influenza hemagglu
- Page 19 and 20: Strains Selected for Fermentation R
- Page 21 and 22: 4-mL Fermentation PhyTip-IMAC Solub
- Page 23 and 24: Downstream Processing Robotic Batch
- Page 25 and 26: Summary Extensive genomics capabili
- Page 27: Acknowledgements: Pfenex Lauren Alc
<strong>HTP</strong> Microchip SDS-CGE<br />
Labchip ® GXII<br />
Protein chip<br />
Schematic layout of the<br />
microchannels of protein chip.<br />
The red channels are filled with<br />
sieving matrix.<br />
LC90<br />
Features of the Labchip GXII:<br />
• Capable of interfacing with plate robotics<br />
• Increased chip stability<br />
• On-line chip priming<br />
• Dry focusing<br />
• Chip RFID reader keeps user informed of onchip<br />
reagent <strong>and</strong> chip lifetime status<br />
• CFR21 Part 11 compliance<br />
• Great improvements in software<br />
Discovery ● Development ● Production<br />
12
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