Fully Automated Sample Processing and HTP ... - PerkinElmer
Fully Automated Sample Processing and HTP ... - PerkinElmer
Fully Automated Sample Processing and HTP ... - PerkinElmer
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<strong>HTP</strong> Growth <strong>and</strong> Expression<br />
2<br />
plasmids<br />
4+ promoters<br />
3 ribosome binding sites<br />
25 secretion leaders<br />
• High throughput parallel processing<br />
• Yield, quality <strong>and</strong> speed create significant advantages<br />
in real <strong>and</strong> opportunity costs<br />
30 chaperone/disulfide bond<br />
isomerase overexpression plasmids<br />
100+ protease clean deletion mutants, multiple deletions<br />
Strain Construction<br />
+<br />
plasmids host strains<br />
Seed<br />
electroporate <strong>and</strong> grow<br />
transformants on<br />
selective media<br />
<strong>HTP</strong> Expression<br />
Inoculate seed<br />
culture into <strong>HTP</strong><br />
medium<br />
Replicate cultures<br />
will be grown for 24<br />
h at 30 °C prior to<br />
induction<br />
Harvest<br />
<strong>and</strong><br />
Analysis<br />
Thous<strong>and</strong>s of strains evaluated in
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