peqGOLD 50 bp DNA-Ladder Orange G - Peqlab

Data Sheet
peqGOLD 50 bp DNA-Ladder Orange G
'improved version'
Lot-No. ........
Concentration
Usage
0.1 mg DNA/ml
1 µl (0.1 µg)/mm Lane
Order-No. 25-2350 1 x 50 µg 1 x 1 ml Orange G Loading buffer for samples (6x)
25-2351 5 x 50 µg 2 x 1 ml Orange G Loading buffer for samples (6x)
Description
peqGOLD 50 bp DNA-Ladder Orange G consists of fragments derived from specially digested DNA
of plasmid pEJ3, which contains pUC, λ phage and Yeast sequences. The marker is shipped 'ready-
to-use'
in Loading buffer (10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.025 % Orange G, 0.005 %
xylene cyanol and 10 % glycerol)
13 Fragments 1000, 900, 800, 700, 600, 500, 400, 300, 250, 200, 150, 100 and 50 base pairs
6x Orange G
Loading buffer
Note
10 mM Tris-HCL (pH 7.6), 0.15 % Orange G, 0.03 % xylene cyanol, 60 % glycerol and 60 mM EDTA
peqGOLD 50 bp DNA-Ladder Orange G is ideal for sizing of unknown DNA fragments in the range
of 50 to 1031 base pairs. Additional weak bands might occur in agarose gels which result from
circular forms of DNA ligation products.
The 'improved version' is characterized by equalized amounts of DNA per band for easier
quantification. The 1031 bp fragment was replaced by a 1000 bp fragment. The 500 bp and the 250
bp fragments were introduced as new reference bands.
Loading
Agarose and non-denaturing Polyacrylamide gels:
peqGOLD 50 bp DNA-Ladder Orange G is ready-to-use and can be applied directly to the gel:
Vortex marker briefly.
Load 1 µl (0.1 µg) DNA-Ladder per 1 mm lane width
Visualize fragments after electrophoretic separation by ethidium bromide-staining. Do not heat before
loading. 50 µg marker are sufficient for approx. 100 lanes (5 mm Lane width).
Peqlab_V0911_E
info@peqlab.com · www.peqlab.com

peqGOLD 50 bp DNA-Ladder Orange G
Quantification
See the graph for the percentage of the bands and the
amount of DNA per band in ng, relating to 0.5 µg loaded
marker. Use the same volume of DNA and marker.
Additionally the concentration of loading buffer in samples
and marker should be equal.
Note
Ethidium bromide migrates contrarily to the DNA during
electrophoresis. Therefore the distribution of ethidium
bromide in the gel is not constant. To ensure equal
distribution of ethidium bromide in the gel add 0.5 mg/l
ethidium bromide to electrophoresis buffer or dye the gel after
the run.
Shipment: blue ice
Storage: + 4 °C/– 20 °C
Stability: up to 6 months at + 4 °C
minimum 18 months at – 20 °C
Peqlab_V0911_E
info@peqlab.com · www.peqlab.com