PEBC Report - Programa de Epigenética y Biología del Cáncer

Selected publications
DNA methylation and asymmetric chromatin structure.
Imprinted genes are typically involved in embryonic growth
and development. Abnormal imprinted gene expression is
one of the most frequent aberrations in carcinogenesis.
Our research aims to understand the controlling mechanisms
involved in genomic imprinting, and how genetic and
epigenetic variation in imprinted genes is associated with
cancer susceptibility and development. We currently have
projects assessing the hierarchical chromatin organisation at
imprinting control regions in leukaemias and solid tumours,
assessing how aberrant histone modifications relate to
DNA-methylation profiles. These projects use state-of-the
art technologies, including 5-mC HPLC, ChIP-seq and custom
DNA-methylation arrays. In addition, using the same
molecular techniques, we are assessing the epigenetic profiles
of babies born following assisted reproductive techniques
to try and understand the epigenetic mechanisms
involved in ART associated loss-of-imprinting.
Monk D, Wagschal A, Arnaud P, Müller PS, Parker-Katiraee L,
Bourc'his D, Scherer SW, Feil R, Stanier P, Moore GE.
Comparative analysis of human chromosome 7q21 and
mouse proximal chromosome 6 reveals a placental-specific
imprinted gene, TFPI2/Tfpi2, which requires EHMT2 and EED
for allelic-silencing. Genome Res. 2008 Aug;18(8):1270-81.
Epub 2008 May 14.
Iglesias-Platas I*, Monk D*, Jebbink J, Buimer M, Boer K, van
der Post J, Hills F, Apostolidou S, Ris-Stalpers C, Stanier P,
Moore GE. STOX1 is not imprinted and is not likely to be
involved in preeclampsia. Nat Genet. 2007 Mar;39(3):279-
80. *These authors contributed equally to this work
Monk D, Arnaud P, Apostolidou S, Hills FA, Kelsey G, Stanier
P, Feil R, Moore GE. Limited evolutionary conservation of
imprinting in the human placenta. Proc Natl Acad Sci USA.
2006 Apr 25;103(17):6623-8.
Monk D, Sanches R, Arnaud P, Apostolidou S, Hills FA, Abu-
Amero S, Murrell A, Friess H, Reik W, Stanier P, Constância
M, Moore GE. Imprinting of IGF2 P0 transcript and novel
alternatively spliced INS-IGF2 isoforms show differences
between mouse and human. Hum Mol Genet. 2006 Apr
15;15(8):1259-69.
Monk D, Smith R, Arnaud P, Preece MA, Stanier P, Beechey
CV, Peters J, Kelsey G, Moore GE. Imprinted methylation profiles
for proximal mouse chromosomes 11 and 7 as revealed
by methylation-sensitive representational difference analysis.
Mamm Genome. 2003 Dec;14(12):805-16.
Monk D, Bentley L, Beechey C, Hitchins M, Peters J, Preece
MA, Stanier P, Moore GE. Characterisation of the growth
regulating gene IMP3, a candidate for Silver-Russell syndrome.
J Med Genet. 2002 Aug;39(8):575-81.
Monk D, Bentley L, Hitchins M, Myler RA, Clayton-Smith J,
Ismail S, Price SM, Preece MA, Stanier P, Moore GE.
Chromosome 7p disruptions in Silver Russell syndrome:
delineating an imprinted candidate gene region. Hum Genet.
2002 Oct;111(4-5):376-87.
Cancer Epigenetics and Biology Symposium
44 28, 29 May 2009, Barcelona