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Printable Test Catalog - OU Medicine

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If Virology is closed use following method for VZ culture only.<br />

1. Unroof vesicle with #15 Bard-Parker scalpel.<br />

2. Scrape the base of the vesicle (obtain basal and parabasal<br />

cells) with a NP/male swab.<br />

3. Break swab in to viral trasnport media.<br />

4. Transport to the lab immediately. Keep refrigerated.<br />

TUBE OR CONTAINER: Viral transport media<br />

SPECIAL HANDLING: Call 271-3586 for collection materials and<br />

questions about specimen collection<br />

TEST AVAILABILITY: Monday-Saturday<br />

TURNAR<strong>OU</strong>ND TIME: 48-96 hours<br />

METHODOLOGY: Shell vial<br />

CPT: 87252; 87254<br />

[VVZVC] VARICELLA ZOSTER CULTURE<br />

SPECIMEN REQUIRED: Refrigerated specimen<br />

COLLECTION INSTRUCTIONS:<br />

1. Pick freshest vesicle<br />

2. Collect culture first, aspirate the fluid from the vesicle<br />

into the tuberculin syringe and inject it into the cell<br />

culture tubes. Obtain collection materials from Virology<br />

M-F 7 am to 5 pm and Saturday 10 am to 12 pm.<br />

If Virology is closed use following method for VZ culture only.<br />

1. Unroof vesicle with #15 Bard-Parker scalpel.<br />

2. Scrape the base of the vesicle (obtain basal and parabasal<br />

cells) with a NP/male swab.<br />

3. Break swab in to viral transport media.<br />

4. Transport to the lab immediately. Keep refrigerated.<br />

S<strong>OU</strong>RCE:<br />

VESICLE FLUID: Collect specimen with viral culture swab;<br />

refrigerate following collection and during transport.<br />

LUNG TISSUE: Submit lung biopsy material in sterile screw-cap<br />

container. Add a small amount of sterile saline to prevent<br />

drying. Do not add formalin; if pathology exam is ordered<br />

also, submit separate specimen.<br />

TUBE OR CONTAINER: Viral transport media<br />

SPECIAL HANDLING: Indicate source of specimen; inclusion of<br />

patient's clinical history and/or differential<br />

diagnostic considerations is very helpful;<br />

Call 271-3586 for collection materials and<br />

questions about specimen collection<br />

ORDERING MODULE: MIC<br />

TEST INCLUDES: VZV culture ONLY<br />

TEST AVAILABILITY: Set up Monday-Saturday<br />

TURNAR<strong>OU</strong>ND TIME: 10 days<br />

REFERENCE RANGE: No growth<br />

METHODOLOGY: Cytopathic Effect (CPE)<br />

CLINICAL USE: Aid in diagnosis of disease caused by varicellazoster<br />

virus (ie, chickenpox and shingles)<br />

CPT: 87252

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