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Printable Test Catalog - OU Medicine

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medium or have the patient gargle 3 mL of sterile saline for<br />

10 seconds; expell the gargle into a sterile container.<br />

This is for a viral culture if the slide collection is poor<br />

or if the FA is negative. The culture is not performed if<br />

the FA is positive.<br />

The aspirate can be put in viral transport medium and used for<br />

culture backup. Culture backup for adults requires a throat swab<br />

or gargle placed in viral transport medium. If FA is positive<br />

the culture is not performed. Select any 2 viruses to be tested<br />

for: RSV, Parainfluenza virus, Influenza A/B, Adenovirus.<br />

Influenza A and B can be tested for separately or pooled.<br />

Draw up 1-2 mL sterile saline into a 3-5 mL syringe. Attach 2<br />

inches of 18-20 guage tubing to syringe tip. Insert tube end<br />

into nostril. Quickly instill saline into nostril. Aspirate the<br />

recoverable nasal specimen. Inject nasal specimen from syringe<br />

into viral transport media tube. Refrigerate following<br />

collection and during transport.<br />

NP swab: Collect with rayon or Dacron wire swab; place swab into<br />

viral transport media tube. Refrigerate following collection<br />

and during transport.<br />

TUBE OR CONTAINER: Viral transport media<br />

TEST AVAILABILLTIY: Monday-Saturday<br />

TURNAR<strong>OU</strong>ND TIME: 24 hours<br />

METHODOLOGY: Direct fluorescent antibody (DFA)<br />

[VPARFAI] PARAINFLUENZA FA + CULTURE<br />

SPECIMEN REQUIRED:<br />

COLLECTION INSTRUCTIONS:<br />

Nasopharyngeal wash+NP:<br />

1. Draw up 1-2 mL of sterile saline into a 3-5 mL syringe.<br />

2. Remove the needle from the syringe and put on a butterfly<br />

with a large tubing. Cut the tubing to a length of 2-3".<br />

3. Place the tubing in the nasopharyngeal passage. Inject the<br />

saline rapidly and draw back rapidly.<br />

4. Inject saline and nasal material into the viral transport<br />

medium. If viral transport medium is not available, the NP<br />

wash may be added to 1.O mL of trypticase soy broth or<br />

sterile saline.<br />

5. After or before doing the wash, use a dacron NP swab to do<br />

a good epithelial cell scraping of the nasopharyngeal area<br />

Place and cut or break the swab into the same vial as the<br />

wash. (Immunofluorescent testing is done on the epithelial<br />

cells the combination of both the wash and nasopharyngeal<br />

scraping are essential to obtain enough cells to perform<br />

the test.)<br />

Alternate NP collection instructions:<br />

1. Insert a type 1 (NP) dacron tipped, aluminum-shafted swab<br />

into nasopharyngeal passage for 1 minute.<br />

2. Immediately following the removal of the swab roll the<br />

swab onto the center of a glass slide. Keep the area<br />

confined to the size of a dime. Do not leave any large

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