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Printable Test Catalog - OU Medicine

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METHODOLOGY: Liquid chromatography/Tandem Mass Spectrometry<br />

(LC-MS/MS)<br />

CLINICAL USE: Diagnose pheochromocytoma; evaluation of hypertension,<br />

diagnosis and follow up neuroblastoma,<br />

ganglioneuroma, and ganglioneuroblastoma<br />

CPT: 82570; 84585<br />

[VMEAFAI] MEASLES VIRUS FA + CULTURE<br />

SPECIMEN REQUIRED:<br />

Nasopharyngeal wash+NP instructions:<br />

1. Draw up 1-2 mL of sterile saline into a 3-5 mL syringe.<br />

2. Remove the needle from the syringe and put on a butterfly<br />

with a large tubing. Cut the tubing to a length of 2-3".<br />

3. Place the tubing in the nasopharyngeal passage. Inject the<br />

saline rapidly and draw back rapidly.<br />

4. Inject saline and nasal material into the viral transport<br />

medium. (If viral transport medium is not available, the NP<br />

wash may be added to 1.O mL of trypticase soy broth or<br />

sterile saline.)<br />

5. After or before doing the wash, use a Dacron NP swab to do a<br />

good epithelial cell scraping of the nasopharyngeal area.<br />

Place and cut or break the swab into the same vial as the<br />

wash. (Immunofluorescent testing is done on the epithelial<br />

cells. The combination of both the wash and nasopharyngeal<br />

scraping are essential to obtain enough cells to perform<br />

the test.)<br />

Alternate NP collection instructions:<br />

1. Insert a type 1 (NP) dacron tipped, aluminum-shafted swab<br />

into nasopharyngeal passage for 1 minute.<br />

2. Immediately following the removal of the swab roll the<br />

swab onto the center of a glass slide. Keep the area<br />

confined to the size of a dime. Do not leave any large<br />

clumps of mucous; spread the specimen out evenly over the<br />

dime size area. (This will make a maximum of 2 slides).<br />

Send to the lab immediately in glass slide holders<br />

3. Repeat step 1 and cut or break the swab into viral transport<br />

medium or have the patient gargle 3 mL of sterile saline for<br />

10 seconds; expell the gargle into a sterile container.<br />

This is for a viral culture if the slide collection is poor<br />

or if the FA is negative. The culture is not performed if<br />

the FA is positive.<br />

TUBE OR CONTAINER: Viral tranport tube<br />

TEST INCLUDES: Measles FA and culture<br />

TEST AVAILABILITY: Monday-Saturday<br />

TURNAR<strong>OU</strong>ND TIME: 1-14 days<br />

METHODOLOGY: Cytopathic effect (CE); Fluorescent antibody (FA)<br />

CPT: 87252; 87283<br />

[VMEASVI] MEASLES SHELL VIAL + CULTURE<br />

SPECIMEN REQUIRED:<br />

Nasopharyngeal wash+NP instructions:

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