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Printable Test Catalog - OU Medicine

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collection and during transport.<br />

NP swab: Collect with rayon or Dacron wire swab; place swab into<br />

viral transport media tube. Refrigerate following collection<br />

and during transport.<br />

TUBE OR CONTAINER: Viral transport media<br />

TEST AVAILABILLTIY: Monday-Saturday<br />

TURNAR<strong>OU</strong>ND TIME: 24 hours<br />

METHODOLOGY: Direct fluorescent antibody (DFA)<br />

[VINASVI] INFLUENZA A SHELL V. + CULTURE<br />

Nasopharyngeal wash+NP instructions:<br />

1. Draw up 1-2 mL of sterile saline into a 3-5 mL syringe.<br />

2. Remove the needle from the syringe and put on a butterfly<br />

with a large tubing. Cut the tubing to a length of 2-3".<br />

3. Place the tubing in the nasopharyngeal passage. Inject the<br />

saline rapidly and draw back rapidly.<br />

4. Inject saline and nasal material into the viral transport<br />

medium. (If viral transport medium is not available, the NP<br />

wash may be added to 1.O mL of trypticase soy broth or<br />

sterile saline.)<br />

5. After or before doing the wash, use a Dacron NP swab to do a<br />

good epithelial cell scraping of the nasopharyngeal area.<br />

Place and cut or break the swab into the same vial as the<br />

wash. (Immunofluorescent testing is done on the epithelial<br />

cells. The combination of both the wash and nasopharyngeal<br />

scraping are essential to obtain enough cells to perform<br />

the test.)<br />

6. Transport immediately<br />

TUBE OR CONTAINER: Viral transport media<br />

TEST INCLUDES: Influenza A Shell Vial, and Viral Respiratory<br />

Culture<br />

TEST AVAILABILITY: Monday-Saturday<br />

TURNAR<strong>OU</strong>ND TIME: 48-96 hours<br />

METHODOLOGY: Shell Vial<br />

CPT: 87254; 87252<br />

[VINAVC] INFLUENZA A CULTURE<br />

SPECIMEN REQUIRED: Any respiratory sample on ice<br />

COLLECTION INSTRUCTIONS:<br />

Nasopharyngeal wash+NP:<br />

1. Draw up 1-2 mL of sterile saline into a 3-5 mL syringe.<br />

2. Remove the needle from the syringe and put on a butterfly<br />

with a large tubing. Cut the tubing to a length of 2-3".<br />

3. Place the tubing in the nasopharyngeal passage. Inject the<br />

saline rapidly and draw back rapidly.<br />

4. Inject saline and nasal material into the viral transport<br />

medium. If viral transport medium is not available, the NP<br />

wash may be added to 1.O mL of trypticase soy broth or<br />

sterile saline.<br />

5. After or before doing the wash, use a dacron NP swab to do

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