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Printable Test Catalog - OU Medicine

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3. Place the tubing in the nasopharyngeal passage. Inject the<br />

saline rapidly and draw back rapidly.<br />

4. Inject saline and nasal material into the viral transport<br />

medium. If viral transport medium is not available, the NP<br />

wash may be added to 1.O mL of trypticase soy broth or<br />

sterile saline.<br />

5. After or before doing the wash, use a dacron NP swab to do<br />

a good epithelial cell scraping of the nasopharyngeal area<br />

Place and cut or break the swab into the same vial as the<br />

wash. (Immunofluorescent testing is done on the epithelial<br />

cells the combination of both the wash and nasopharyngeal<br />

scraping are essential to obtain enough cells to perform<br />

the test.)<br />

Alternate NP collection instructions:<br />

1. Insert a type 1 (NP) dacron tipped, aluminum-shafted swab<br />

into nasopharyngeal passage for 1 minute.<br />

2. Immediately following the removal of the swab roll the<br />

swab onto the center of a glass slide. Keep the area<br />

confined to the size of a dime. Do not leave any large<br />

clumps of mucous; spread the specimen out evenly over the<br />

dime size area. (This will make a maximum of 2 slides).<br />

Send to the lab immediately in glass slide holders<br />

3. Repeat step 1 and cut or break the swab into viral transport<br />

medium or have the patient gargle 3mL of sterile<br />

saline for 10 seconds; expell the gargle into a sterile<br />

container. This is for a viral culture if the slide<br />

collection is poor or if the FA is negative. The culture<br />

is not performed if the FA is positive.<br />

TUBE OR CONTAINER: Viral transport tube<br />

ORDERING MODULE: MIC<br />

TEST INCLUDES: RSV; Parainfluenza Pool; Influenza A & B,<br />

Adenovirus and hMetapnuemovirus<br />

TEST AVAILABILITY: Daily<br />

TURNAR<strong>OU</strong>ND TIME: 24 hours<br />

REFERENCE RANGE: Negative<br />

METHODOLOGY: Direct fluorescent antibody (DFA)<br />

CPT: 87252; 87260; 87275; 87276; 87279; 87280<br />

[VGCCAB] Voltage-Gated Calcium Channel Ab<br />

SPECIMEN REQUIRED: Refrigerated serum<br />

TUBE OR CONTAINER: Red or Gold<br />

SPECIAL HANDLING: Separate serum from cells within 1 hour of<br />

collection<br />

SPECIMEN VOLUME: 0.2 mL<br />

MINIMUM VOLUME: 0.1 (this volume does not allow for repeat<br />

testing)<br />

TEST AVAILABILITY: Daily<br />

REFERENCE RANGE: OTHER NAMES: Lambert-Eaton Myasthenic Syndrome<br />

Antibody; LEMS<br />

Autoantibody; VGCCA; VGCC Antibody<br />

METHODOLOGY: Radioimmunoassay (RIA)

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