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Fluorescence in vivo imaging for oncology<br />
Justin Teissié<br />
IPBS CNRS UMR5089, 205 route de narbonne 31077 Toulouse (France);<br />
Justin.teissie@ipbs.fr<br />
Following and quantifying the expression of reporter gene expression in vivo is<br />
very important to monitor the expression of therapeutic genes in targeted tissues in<br />
disease models, to follow the fate of tumour cells, and/or to assess the effectiveness<br />
of systems of gene therapy delivery. Gene expression of fluorescent proteins can<br />
be detected directly on living animals by simply observing the associated optical<br />
signals by mean of a cooled charged-coupled device (CCCD) camera. More accurate<br />
resolution can be obtained with more sophisticated technologies. Time- course and<br />
quasi-quantitative monitoring of the expression can be obtained on a given animal<br />
and followed on a large time window. The present lecture describes the physical<br />
and technological methodologies and associated problems of in vivo optical imaging.<br />
Several examples of in vivo detection for oncology purposes are described.<br />
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