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Tissue swelling at the site of electroporation, which spontaneously resolved within 2<br />
to 3 days after the procedure, was the only observed side-effect of the procedure.<br />
In conclusion, according to our study, it is possible to achieve good transfection<br />
efficiency of canine skeletal muscle using two different EP protocols, either<br />
combination of 1 HV (600 V/cm, 100 µs) pulse, followed by 4 LV pulses (80 V/cm,<br />
100 ms, 1Hz), or 8 LV pulses (200 V/cm, 20 ms, 1Hz), resulting in expression of<br />
transgene, lasting at least 7 days.<br />
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