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Paper Conservation: Decisions & Compromises

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Fig. 2: Chromatogram of the first Sample showing the presence of luteolin and apigenin (or genistein)<br />

The third sample was undyed and from the same<br />

book which appeared to show a peak for luteolin<br />

at m/z 285, retention time 5.82, this was unexpected,<br />

and however, the apigenin or genistein<br />

peak was not observed in the undyed sample.<br />

3.3. Results from the microbial study, by using<br />

Cork Borer technique<br />

Clear zones of inhibition were observed on the<br />

agar plates containing the three chosen strains<br />

of bacteria after incubation around the punched<br />

hole containing safflower dye solution made<br />

from a traditional recipe and including lemon<br />

juice (Fig. 3). The diameter of the clear zones was<br />

measured.<br />

4. Discussion<br />

4.1. Four yellow natural dyes (turmeric, weld,<br />

safflower and saffron) were selected for special<br />

study because of the prevalence of yellow dyed<br />

endpapers in Egyptian medieval manuscript<br />

books (Fig. 4), noted during extensive handling<br />

of collections and because their application was<br />

widespread during most historical periods (Baker<br />

1995; Loveday 2001; Graaff 2004; Cardon 2007).<br />

To date, there is no conclusive evidence from<br />

primary texts, modern scientific publication or<br />

any other explanation as to whether yellow dyes<br />

Fig. 3: Cork borer test for the effect of safflower dye against<br />

Bacillus subtilis grown on nutrient agar medium<br />

were used for any specific purpose.<br />

In the Islamic mediaeval era, papermakers were<br />

producing paper ranging in colour from cream<br />

to dark cream and either grey or off-white in<br />

tone according to its production inputs, there is<br />

no record of colour being added to the pulp in<br />

ICOM-CC Graphic Documents Working Group Interim Meeting | Vienna 17 – 19 April 2013<br />

63

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