Surface Modification of Cellulose Acetate with Cutinase and ...

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Enzymatic Hydolysis of Wool with a Genetically Modified Subtilisin E of felting and pilling than samples treated with chimeric enzyme. In fact it seems that yarns treated with subtilisin-VPAVG220 felted even less than the control samples what emphasize the idea that, due to its size, the hydrolytic activity of chimeric enzyme is restricted to the surface of wool yarns and also that the elastomeric polymer VPAVG220 can provide some kind of protection of yarns against excessive damage after 3 cicles of washing. Control without enzyme (A) Esperase (B) Subtilisin-GAG220 (C) Figure 4. Visual damages on wool yarns after 3 cycles washing in a Rotawash machine. A) Wool yarns without enzyme; B) wool yarns treated with commercial subtilisin and C) wool yarns treated with subtilisin-VPAVG220. 4. Conclusion Without pre-treatment Scouring washing Scouring washing and bleaching In this work we have achieved the production of a recombinant high molecular weigh subtilisin through the fusion of subtilisin E DNA sequence with a sequence that codifies to an elastomeric polymer. The effect of chimeric high molecular weight subtilisin on wool yarns was compared to the commercial Esperase. It was found that, as already expected, due to its small size, the commercial subtilisin is able to penetrate inside the wool cortex, damaging the fibre. 213
Subchapter 3.3 Chimeric subtilisin-VPAVG220, the high molecular weight enzyme, hydrolyzed just the cuticle layer of wool. Yarns treated with this enzyme presented higher tensile strength and lower felting. The recombinant hight molecular weight enzyme can be a promissing economical and ecological alternative to the tradicional chlorine treatment. 214
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Universidade do Minho Escola de Ci
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É AUTORIZADA A REPRODUÇÃO PARCIA
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Acknowledgments/ Agradecimentos
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Às 2 mosqueteiras ausentes, Joana
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Abstract The challenges facing the
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Resumo
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Resumo Os desafios que a indústria
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molecular ficou retida à superfíc
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Table of contents Acknowledgments /
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CATs- Catalases CBD- Carbohydrate b
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Chapter 1 General Introduction: App
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General Introduction: Application o
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1. Biotechnology in textile industr
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3. Role of enzymes in textile indus
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7. Serine proteases: subtilisins Ge
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12.1 Decolourization of dyes and te
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References General Introduction: Ap
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Chapter 2 Surface Modification of S
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Subchapter 2.1 52
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Subchapter 2.1 major material of co
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Subchapter 2.1 End uses include swe
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Subchapter 2.1 References Battistel
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Subchapter 2.1 60
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Subchapter 2.2 62
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Subchapter 2.2 Abstract Cutinase fr
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Subchapter 2.2 2. Materials and met
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Subchapter 2.2 Scheme 1. Thermodyna
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Subchapter 2.2 14000 rpm at 4 ºC.
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Subchapter 2.2 3. Results and discu
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Mutation Subchapter 2.2 Table III.
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Subchapter 2.2 Table IV. Hydrophobi
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Subchapter 2.2 References Ansaldi M
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Subchapter 2.2 80
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Subchapter 2.3 82
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Subchapter 2.3 Abstract Two polyami
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Subchapter 2.3 aliphatic polyester.
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Subchapter 2.3 2.3. Determination o
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Subchapter 2.3 2.7. Enzymatic hydro
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Subchapter 2.3 The crystallinity va
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Subchapter 2.3 Table I. Protein ads
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Subchapter 2.3 In the same set of e
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Subchapter 2.3 98 Protein in soluti
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Subchapter 2.3 100 Protein in solut
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Subchapter 2.3 Table II. Time of wa
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Subchapter 2.3 104 Abs 60 50 40 30
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Subchapter 2.3 Acknowledgments The
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Subchapter 2.3 Lau Y, Bruice C. 199
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Subchapter 2.4 110
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Subchapter 2.4 Abstract The effect
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Subchapter 2.4 A balance between en
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Subchapter 2.4 flask with stirring
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Subchapter 2.4 118 TPA (mM) TPA (mM
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Subchapter 2.4 of native cutinase a
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Subchapter 2.4 122 K/S Increase (%)
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Subchapter 2.4 References Araújo R
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Subchapter 2.4 126
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Surface Modification of Cellulose A
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Chapter 3 Enzymatic Modification of
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SubChapter 3.1 Introduction
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Page 198 and 199: Strategies Towards the Functionaliz
Page 200 and 201: 1. Introduction Strategies Towards
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Page 224 and 225: Subchapter 3.3 Enzymatic Hydolysis
Page 226 and 227: Enzymatic Hydolysis of Wool with a
Page 228 and 229: 1. Introduction Enzymatic Hydolysis
Page 238 and 239: References Enzymatic Hydolysis of W
Page 240 and 241: Chapter 4 General Discussion and Fu
Page 242 and 243: General Discussion and Future persp
Page 244 and 245: General Discussion and Future Persp
Page 246 and 247: References General Discussion and F
Page 248 and 249: Appendix
Page 250 and 251: SOB TE buffer Autoclave Tryptone 2%
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