Surface Modification of Cellulose Acetate with Cutinase and ...

More documents

Recommendations

Info

Enzymatic Hydolysis of Wool with a Genetically Modified Subtilisin E Enzymatic Hydrolysis of Wool with a Genetically Modified Subtilisin E RITA ARAÚJO 1 , RAUL MACHADO 1 , CARLA SILVA 2 , ARTUR CAVACO- PAULO 2 *, MARGARIDA CASAL 1 1 Center of Environmental and Molecular Biology, Department of Biology, University of Minho, Campus of Gualtar, 4710-057 Braga, Portugal 2 Center of Textile Engineering, University of Minho, Campus of Azurém, 4800-058 Guimarães, Portugal Keywords: Subtilisin E, protein engineering, protein-based polymer, wool hydrolysis 203
Subchapter 3.3 Abstract In this work, we propose the construction of a novel high molecular weight subtilisin based on the fusion of prosubtitlin E DNA sequence from Bacillus subtilis with a DNA sequence that codifies to an elastin-like polymer. Wool yarns were treated with both commercial and chimeric subtilisins. It was shown that using the chimeric subtilisin there was a significant reduction of felting, pilling and tensile strength loss of wool yarns since the hydrolysis is restricted to the cuticle of wool. The results stated here are of great importance once it is reported for the first time the microbial production of a chimeric high molecular weight protease for wool surface hydrolysis. This novel process of enzymatic-controlled hydrolysis overcomes the unrestrained diffusion and extended fibre damage which is the major obstacle on the use of enzymes for wool finishing applications. 204
Page 1 and 2:
Universidade do Minho Escola de Ci
Page 3 and 4:
É AUTORIZADA A REPRODUÇÃO PARCIA
Page 6:
Acknowledgments/ Agradecimentos
Page 9 and 10:
Às 2 mosqueteiras ausentes, Joana
Page 12 and 13:
Abstract The challenges facing the
Page 14 and 15:
Resumo
Page 16 and 17:
Resumo Os desafios que a indústria
Page 18 and 19:
molecular ficou retida à superfíc
Page 20 and 21:
Table of contents Acknowledgments /
Page 22 and 23:
CATs- Catalases CBD- Carbohydrate b
Page 24 and 25:
Chapter 1 General Introduction: App
Page 26 and 27:
General Introduction: Application o
Page 28 and 29:
1. Biotechnology in textile industr
Page 30 and 31:
3. Role of enzymes in textile indus
Page 32 and 33:
Page 34 and 35:
Page 36 and 37:
Page 38 and 39:
Page 40 and 41:
7. Serine proteases: subtilisins Ge
Page 42 and 43:
Page 44 and 45:
Page 46 and 47:
Page 48 and 49:
Page 50 and 51:
Page 52 and 53:
12.1 Decolourization of dyes and te
Page 54 and 55:
Page 56 and 57:
References General Introduction: Ap
Page 58 and 59:
Page 60 and 61:
Page 62 and 63:
Page 64 and 65:
Page 66 and 67:
Page 68 and 69:
Page 70 and 71:
Page 72:
Chapter 2 Surface Modification of S
Page 75 and 76:
Subchapter 2.1 52
Page 77 and 78:
Subchapter 2.1 major material of co
Page 79 and 80:
Subchapter 2.1 End uses include swe
Page 81 and 82:
Subchapter 2.1 References Battistel
Page 83 and 84:
Subchapter 2.1 60
Page 85 and 86:
Subchapter 2.2 62
Page 87 and 88:
Subchapter 2.2 Abstract Cutinase fr
Page 89 and 90:
Subchapter 2.2 2. Materials and met
Page 91 and 92:
Subchapter 2.2 Scheme 1. Thermodyna
Page 93 and 94:
Subchapter 2.2 14000 rpm at 4 ºC.
Page 95 and 96:
Subchapter 2.2 3. Results and discu
Page 97 and 98:
Mutation Subchapter 2.2 Table III.
Page 99 and 100:
Subchapter 2.2 Table IV. Hydrophobi
Page 101 and 102:
Subchapter 2.2 References Ansaldi M
Page 103 and 104:
Subchapter 2.2 80
Page 105 and 106:
Subchapter 2.3 82
Page 107 and 108:
Subchapter 2.3 Abstract Two polyami
Page 109 and 110:
Subchapter 2.3 aliphatic polyester.
Page 111 and 112:
Subchapter 2.3 2.3. Determination o
Page 113 and 114:
Subchapter 2.3 2.7. Enzymatic hydro
Page 115 and 116:
Subchapter 2.3 The crystallinity va
Page 117 and 118:
Subchapter 2.3 Table I. Protein ads
Page 119 and 120:
Subchapter 2.3 In the same set of e
Page 121 and 122:
Subchapter 2.3 98 Protein in soluti
Page 123 and 124:
Subchapter 2.3 100 Protein in solut
Page 125 and 126:
Subchapter 2.3 Table II. Time of wa
Page 127 and 128:
Subchapter 2.3 104 Abs 60 50 40 30
Page 129 and 130:
Subchapter 2.3 Acknowledgments The
Page 131 and 132:
Subchapter 2.3 Lau Y, Bruice C. 199
Page 133 and 134:
Subchapter 2.4 110
Page 135 and 136:
Subchapter 2.4 Abstract The effect
Page 137 and 138:
Subchapter 2.4 A balance between en
Page 139 and 140:
Subchapter 2.4 flask with stirring
Page 141 and 142:
Subchapter 2.4 118 TPA (mM) TPA (mM
Page 143 and 144:
Subchapter 2.4 of native cutinase a
Page 145 and 146:
Subchapter 2.4 122 K/S Increase (%)
Page 147 and 148:
Subchapter 2.4 References Araújo R
Page 149 and 150:
Subchapter 2.4 126
Page 152 and 153:
Surface Modification of Cellulose A
Page 154 and 155:
Page 156 and 157:
Page 158 and 159:
Page 160 and 161:
Page 162 and 163:
Page 164 and 165:
Page 166 and 167:
Page 168 and 169:
Page 170 and 171:
Page 172 and 173:
Page 174 and 175:
Page 176 and 177: Surface Modification of Cellulose A
Page 182 and 183: Chapter 3 Enzymatic Modification of
Page 184 and 185: SubChapter 3.1 Introduction
Page 186 and 187: 1. Properties of Wool Enzymatic Mod
Page 188 and 189: Enzymatic Modification of Wool Surf
Page 190 and 191: 3.3. Bleaching Enzymatic Modificati
Page 196 and 197: Subchapter 3.2 Strategies Towards t
Page 198 and 199: Strategies Towards the Functionaliz
Page 200 and 201: 1. Introduction Strategies Towards
Page 218 and 219: 4. Discussion Strategies Towards th
Page 220 and 221: References Strategies Towards the F
Page 224 and 225: Subchapter 3.3 Enzymatic Hydolysis
Page 228 and 229: 1. Introduction Enzymatic Hydolysis
Page 230 and 231: Enzymatic Hydolysis of Wool with a
Page 238 and 239: References Enzymatic Hydolysis of W
Page 240 and 241: Chapter 4 General Discussion and Fu
Page 242 and 243: General Discussion and Future persp
Page 244 and 245: General Discussion and Future Persp
Page 246 and 247: References General Discussion and F
Page 248 and 249: Appendix
Page 250 and 251: SOB TE buffer Autoclave Tryptone 2%
Page 252: Dissolve the nucleic acids in 30 μ
show all

Surface Modification of Cellulose Acetate with Cutinase and ...

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?