Surface Modification of Cellulose Acetate with Cutinase and ...
Surface Modification of Cellulose Acetate with Cutinase and ...
Surface Modification of Cellulose Acetate with Cutinase and ...
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
Subchapter 3.2<br />
190<br />
Soluble fraction Insoluble fraction Soluble fraction Insoluble fraction<br />
MW 1 2 3 MW 4 5 6 MW 7 8 9 10 11 12<br />
Figure 3. SDS-PAGE <strong>of</strong> proteins from E. coli BL21(DE3) cells grown at 30 ºC,<br />
induced <strong>with</strong> IPTG 0.5 mM at 18 ºC <strong>and</strong> grown at 30 ºC, induced <strong>with</strong> IPTG 0.3 mM at<br />
4 ºC. Lanes 1, 4, 7 <strong>and</strong> 10: pET25-prosubtilisinE. Lanes 2, 5, 8 <strong>and</strong> 11: pET25pro2subtilisinE.<br />
Lanes 3, 6, 9 <strong>and</strong> 12: pET25-pro4subtilisinE. MW: SDS-PAGE<br />
St<strong>and</strong>ard, Broad Range (Bio-Rad). The solid arrowheads indicate the position <strong>of</strong><br />
recombinant proteins.<br />
MW 1 2 3 MW 4 5 6 MW 7 8 9 10 11 12<br />
Figure 4. SDS-PAGE <strong>of</strong> proteins from E. coli BL21(DE3) cells grown at 25 ºC,<br />
induced <strong>with</strong> IPTG 0.5 mM at 18 ºC <strong>and</strong> grown at 25 ºC, induced <strong>with</strong> IPTG 0.3 mM at<br />
4 ºC. Lanes 1, 4, 7 <strong>and</strong> 10: pET25-prosubtilisinE. Lanes 2, 5, 8 <strong>and</strong> 11: pET25-<br />
pro2subtilisinE. Lanes 3, 6, 9 <strong>and</strong> 12: pET25-pro4subtilisinE. MW: SDS-PAGE<br />
St<strong>and</strong>ard, Broad Range (Bio-Rad). The solid arrowheads indicate the position <strong>of</strong><br />
recombinant proteins.<br />
Soluble fraction Insoluble fraction Soluble fraction Insoluble fraction
Change language