Surface Modification of Cellulose Acetate with Cutinase and ...

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Table of contents Acknowledgments / Agradecimentos ........................................................................... V Abstract..........................................................................................................................IX Resumo........................................................................................................................XIII Table of contents ........................................................................................................XIX List of Abbreviations and symbols...........................................................................XXI Chapter 1 General Introduction: Application of Enzymes for Textile Fibres Processing................. 1 Chapter 2 Surface Modification of Synthetic Fibres....................................................................... 49 Subchapter 2.1 Introduction..............................................................................................................................51 Subchapter 2.2 Tailoring Cutinase Activity Towards Polyethylene Terephthalate and Polyamide 6.6 Fibers .............................................................................................................61 Subchapter 2.3 Influence of Mechanical Agitation on Cutinases and Protease Activity Toward Polyamide Substrates..................................................................................................81 Subchapter 2.4 Effect of the Agitation on the Adsorption and Hydrolytic Efficiency of Cutinases on Polyethylene Terephthalate Fibres...................................................................109 Subchapter 2.5 Surface Modification of Cellulose Acetate with Cutinase and Cutinase Fused to Carbohydrate-binding Modules ..............................................................................127 Chapter 3 Enzymatic Modification of Wool Surface.................................................................... 159 Subchapter 3.1 Introduction............................................................................................................................161 xix
Subchapter 3.2 Strategies Towards the Functionalization of Bacillus subtilis Subtilisin E for Wool Finishing Applications ........................................................................................... 173 Subchapter 3.3 Enzymatic Hydolysis of Wool with a Genetically Modified Subtilisin E............................... 201 Chapter 4 General Discussion and Future Perspectives ...............................................................217 Appendix ......................................................................................................................225 LIST OF ABBREVIATIONS AND SYMBOLS AFM– Atomic force microscopy Ala/A– Alanine Asn/N– Aspartate ATR– Attenuated total reflectance BOD– biological oxygen demand BSA– Bovine serum albumine xx
Page 1 and 2: Universidade do Minho Escola de Ci
Page 3 and 4: É AUTORIZADA A REPRODUÇÃO PARCIA
Page 6: Acknowledgments/ Agradecimentos
Page 9 and 10: Às 2 mosqueteiras ausentes, Joana
Page 12 and 13: Abstract The challenges facing the
Page 14 and 15: Resumo
Page 16 and 17: Resumo Os desafios que a indústria
Page 18 and 19: molecular ficou retida à superfíc
Page 22 and 23: CATs- Catalases CBD- Carbohydrate b
Page 24 and 25: Chapter 1 General Introduction: App
Page 26 and 27: General Introduction: Application o
Page 28 and 29: 1. Biotechnology in textile industr
Page 30 and 31: 3. Role of enzymes in textile indus
Page 40 and 41: 7. Serine proteases: subtilisins Ge
Page 52 and 53: 12.1 Decolourization of dyes and te
Page 56 and 57: References General Introduction: Ap
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General Introduction: Application o
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Chapter 2 Surface Modification of S
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Subchapter 2.1 52
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Subchapter 2.1 major material of co
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Subchapter 2.1 End uses include swe
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Subchapter 2.1 References Battistel
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Subchapter 2.1 60
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Subchapter 2.2 62
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Subchapter 2.2 Abstract Cutinase fr
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Subchapter 2.2 2. Materials and met
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Subchapter 2.2 Scheme 1. Thermodyna
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Subchapter 2.2 14000 rpm at 4 ºC.
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Subchapter 2.2 3. Results and discu
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Mutation Subchapter 2.2 Table III.
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Subchapter 2.2 Table IV. Hydrophobi
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Subchapter 2.2 References Ansaldi M
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Subchapter 2.2 80
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Subchapter 2.3 82
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Subchapter 2.3 Abstract Two polyami
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Subchapter 2.3 aliphatic polyester.
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Subchapter 2.3 2.3. Determination o
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Subchapter 2.3 2.7. Enzymatic hydro
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Subchapter 2.3 The crystallinity va
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Subchapter 2.3 Table I. Protein ads
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Subchapter 2.3 In the same set of e
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Subchapter 2.3 98 Protein in soluti
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Subchapter 2.3 100 Protein in solut
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Subchapter 2.3 Table II. Time of wa
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Subchapter 2.3 104 Abs 60 50 40 30
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Subchapter 2.3 Acknowledgments The
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Subchapter 2.3 Lau Y, Bruice C. 199
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Subchapter 2.4 110
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Subchapter 2.4 Abstract The effect
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Subchapter 2.4 A balance between en
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Subchapter 2.4 flask with stirring
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Subchapter 2.4 118 TPA (mM) TPA (mM
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Subchapter 2.4 of native cutinase a
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Subchapter 2.4 122 K/S Increase (%)
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Subchapter 2.4 References Araújo R
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Subchapter 2.4 126
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Surface Modification of Cellulose A
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Chapter 3 Enzymatic Modification of
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SubChapter 3.1 Introduction
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1. Properties of Wool Enzymatic Mod
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Enzymatic Modification of Wool Surf
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3.3. Bleaching Enzymatic Modificati
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Subchapter 3.2 Strategies Towards t
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Strategies Towards the Functionaliz
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1. Introduction Strategies Towards
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4. Discussion Strategies Towards th
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References Strategies Towards the F
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Subchapter 3.3 Enzymatic Hydolysis
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Enzymatic Hydolysis of Wool with a
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1. Introduction Enzymatic Hydolysis
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References Enzymatic Hydolysis of W
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Chapter 4 General Discussion and Fu
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General Discussion and Future persp
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General Discussion and Future Persp
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References General Discussion and F
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Appendix
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SOB TE buffer Autoclave Tryptone 2%
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Dissolve the nucleic acids in 30 μ
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