Surface Modification of Cellulose Acetate with Cutinase and ...

Surface Modification of Cellulose Acetate with Cutinase and Cutinase Fused to Carbohydrate-binding Modules
Figure 4. DRIFT spectra showing the carbonyl group stretching band of (A) CDA and
(B) CDT controls and samples. The samples (2% w/v) were treated with 25 U mL -1 of
cutinase, at pH 8 and 30º C, for 24 hours. The controls were treated under the same
conditions but without enzyme.
After the fabric samples and controls were competitively stained, cross sections of
fibres were made and observed by Fluorescence microscopy to assess the diffusion of
the dye inside the fibre and indirectly to confirm the surface action of cutinase.
The inherent affinity of Remazol Reactive Blue R for CDA did not allow any
significant difference in the dye distribution in the fibres (results not showed). The
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