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Surface Modification of Cellulose Acetate with Cutinase and ...

Surface Modification of Cellulose Acetate with Cutinase and ...

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Subchapter 2.5<br />

Figure 3. Progress curves for the formation <strong>of</strong> hydroxyl groups at the surface <strong>of</strong> fibres,<br />

measured as relative increase in K/S values, <strong>and</strong> protein adsorption for the (A) CDA <strong>and</strong><br />

(B) CTA. All the samples (1% w/v) were treated <strong>with</strong> 50 U mL -1 <strong>of</strong> cutinase, at pH 8<br />

<strong>and</strong> 30º C. The controls were treated under the same conditions except for the enzyme.<br />

Samples <strong>and</strong> controls were competitively dyed at 60º C. The relative increase in K/S is<br />

calculated as<br />

as<br />

144<br />

( P0<br />

h − Pt<br />

)<br />

P<br />

( K / S − K / S<br />

0h<br />

enzyme<br />

control<br />

)<br />

K / S<br />

control<br />

(%), where P is the total protein in solution.<br />

(%) <strong>and</strong> the relative protein adsorption

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