Surface Modification of Cellulose Acetate with Cutinase and ...

More documents

Recommendations

Info

Subchapter 2.5 Surface Modification of Cellulose Acetate with Cutinase and Cutinase Fused to Carbohydratebinding Modules The work presented in this chapter has been submitted: Matamá T, Araújo R, Gubitz GM, Casal M, Cavaco-Paulo A. 2008. Surface modification of cellulose acetate with cutinase and cutinase fused to carbohydrate-binding modules. Biotechnology & Bioengineering (submitted)
Page 1 and 2:
Universidade do Minho Escola de Ci
Page 3 and 4:
É AUTORIZADA A REPRODUÇÃO PARCIA
Page 6:
Acknowledgments/ Agradecimentos
Page 9 and 10:
Às 2 mosqueteiras ausentes, Joana
Page 12 and 13:
Abstract The challenges facing the
Page 14 and 15:
Resumo
Page 16 and 17:
Resumo Os desafios que a indústria
Page 18 and 19:
molecular ficou retida à superfíc
Page 20 and 21:
Table of contents Acknowledgments /
Page 22 and 23:
CATs- Catalases CBD- Carbohydrate b
Page 24 and 25:
Chapter 1 General Introduction: App
Page 26 and 27:
General Introduction: Application o
Page 28 and 29:
1. Biotechnology in textile industr
Page 30 and 31:
3. Role of enzymes in textile indus
Page 32 and 33:
Page 34 and 35:
Page 36 and 37:
Page 38 and 39:
Page 40 and 41:
7. Serine proteases: subtilisins Ge
Page 42 and 43:
Page 44 and 45:
Page 46 and 47:
Page 48 and 49:
Page 50 and 51:
Page 52 and 53:
12.1 Decolourization of dyes and te
Page 54 and 55:
Page 56 and 57:
References General Introduction: Ap
Page 58 and 59:
Page 60 and 61:
Page 62 and 63:
Page 64 and 65:
Page 66 and 67:
Page 68 and 69:
Page 70 and 71:
Page 72:
Chapter 2 Surface Modification of S
Page 75 and 76:
Subchapter 2.1 52
Page 77 and 78:
Subchapter 2.1 major material of co
Page 79 and 80:
Subchapter 2.1 End uses include swe
Page 81 and 82:
Subchapter 2.1 References Battistel
Page 83 and 84:
Subchapter 2.1 60
Page 85 and 86:
Subchapter 2.2 62
Page 87 and 88:
Subchapter 2.2 Abstract Cutinase fr
Page 89 and 90:
Subchapter 2.2 2. Materials and met
Page 91 and 92:
Subchapter 2.2 Scheme 1. Thermodyna
Page 93 and 94:
Subchapter 2.2 14000 rpm at 4 ºC.
Page 95 and 96:
Subchapter 2.2 3. Results and discu
Page 97 and 98:
Mutation Subchapter 2.2 Table III.
Page 99 and 100: Subchapter 2.2 Table IV. Hydrophobi
Page 101 and 102: Subchapter 2.2 References Ansaldi M
Page 103 and 104: Subchapter 2.2 80
Page 107 and 108: Subchapter 2.3 Abstract Two polyami
Page 109 and 110: Subchapter 2.3 aliphatic polyester.
Page 111 and 112: Subchapter 2.3 2.3. Determination o
Page 113 and 114: Subchapter 2.3 2.7. Enzymatic hydro
Page 115 and 116: Subchapter 2.3 The crystallinity va
Page 117 and 118: Subchapter 2.3 Table I. Protein ads
Page 119 and 120: Subchapter 2.3 In the same set of e
Page 121 and 122: Subchapter 2.3 98 Protein in soluti
Page 123 and 124: Subchapter 2.3 100 Protein in solut
Page 125 and 126: Subchapter 2.3 Table II. Time of wa
Page 127 and 128: Subchapter 2.3 104 Abs 60 50 40 30
Page 129 and 130: Subchapter 2.3 Acknowledgments The
Page 131 and 132: Subchapter 2.3 Lau Y, Bruice C. 199
Page 135 and 136: Subchapter 2.4 Abstract The effect
Page 137 and 138: Subchapter 2.4 A balance between en
Page 139 and 140: Subchapter 2.4 flask with stirring
Page 141 and 142: Subchapter 2.4 118 TPA (mM) TPA (mM
Page 143 and 144: Subchapter 2.4 of native cutinase a
Page 145 and 146: Subchapter 2.4 122 K/S Increase (%)
Page 147 and 148: Subchapter 2.4 References Araújo R
Page 149: Subchapter 2.4 126
Page 153 and 154: Subchapter 2.5 Abstract The surface
Page 155 and 156: Subchapter 2.5 identified (Samios,
Page 157 and 158: Subchapter 2.5 p-nitrophenol were u
Page 159 and 160: Subchapter 2.5 2.7. Fluorescein Iso
Page 161 and 162: Subchapter 2.5 sCBMCBHI. The plasmi
Page 163 and 164: Subchapter 2.5 reagents and analysi
Page 165 and 166: Subchapter 2.5 The acetic acid rele
Page 167 and 168: Subchapter 2.5 Figure 3. Progress c
Page 169 and 170: Subchapter 2.5 treatment medium of
Page 171 and 172: Subchapter 2.5 differences between
Page 173 and 174: Subchapter 2.5 spacers was studied
Page 175 and 176: Subchapter 2.5 Figure 7. Protein ad
Page 177 and 178: Subchapter 2.5 Acknowledgements Thi
Page 179 and 180: Subchapter 2.5 Guebitz GM, Cavaco-P
Page 181 and 182: Subchapter 2.5 Zugenmaier P. 2004.
Page 183 and 184: Chapter 3 160
Page 187 and 188: Subchapter 3.1 Wool is a complex na
Page 189 and 190: Subchapter 3.1 The cortex comprises
Page 191 and 192: Subchapter 3.1 3.6. Felting and shr
Page 193 and 194: Subchapter 3.1 References Brack N,
Page 195 and 196: 172
Page 199 and 200: Subchapter 3.2 Abstract Subtilisin
Page 201 and 202:
Subchapter 3.2 removal of the prope
Page 203 and 204:
Subchapter 3.2 as, the fermentation
Page 205 and 206:
Subchapter 3.2 Bgl II pGEM 182 Rest
Page 207 and 208:
Subchapter 3.2 Table II. Heterologo
Page 209 and 210:
Subchapter 3.2 on ice and centrifug
Page 211 and 212:
Subchapter 3.2 activity is defined
Page 213 and 214:
Subchapter 3.2 190 Soluble fraction
Page 215 and 216:
Subchapter 3.2 Figure 5. SDS-PAGE o
Page 217 and 218:
Subchapter 3.2 conditions described
Page 219 and 220:
Subchapter 3.2 coli insoluble prote
Page 221 and 222:
Subchapter 3.2 Kumar CG, Takagi H.
Page 223 and 224:
Subchapter 3.2 Wong SL, Doi RH. 198
Page 225 and 226:
Subchapter 3.3 202
Page 227 and 228:
Subchapter 3.3 Abstract In this wor
Page 229 and 230:
Subchapter 3.3 2. Materials and Met
Page 231 and 232:
Subchapter 3.3 amount of enzyme tha
Page 233 and 234:
Subchapter 3.3 in the cellular lysa
Page 235 and 236:
Subchapter 3.3 The maximum tensile
Page 237 and 238:
Subchapter 3.3 Chimeric subtilisin-
Page 239 and 240:
Subchapter 3.3 Trabbic-Carlson K, L
Page 241 and 242:
Chapter 4 218
Page 243 and 244:
Chapter 4 quality of the fibres cau
Page 245 and 246:
Chapter 4 other approache describes
Page 247 and 248:
Chapter 4 Silva C, Araújo R, Casal
Page 249 and 250:
Appendix I Restriction enzyme(s) di
Page 251 and 252:
Heat-shock the tubes in a thermoblo
Page 254:
Este trabalho foi co-financiado pel
show all

Surface Modification of Cellulose Acetate with Cutinase and ...

Create successful ePaper yourself

Delete template?

Save as template?