Surface Modification of Cellulose Acetate with Cutinase and ...

Subchapter 2.3
98
Protein in solution (%)
105
100
95
90
85
80
75
70
65
60
55
50
45
0,0 0,5 1,0 1,5 2,0 2,5 3,0 3,5 4,0 4,5
Time of incubation (h)
Native without discs
L182A without discs
Native with discs
L182A with discs
Figure 4 a). Protein content in the liquor treatment after 4 hours of incubation with
native (282 U mL -1 ) and cutinase mutant (1524 U mL -1 ).
[Amines] (mM)
0,22
0,20
0,18
0,16
0,14
0,12
0,10
0,08
0,06
Native without discs
0,04
L182A without discs
0,02
0,00
Native with discs
L182A with discs
0,0 0,5 1,0 1,5 2,0 2,5 3,0 3,5 4,0 4,5
Time of incubation (h)
Figure 4 b). Amino groups concentration in the liquor treatment after 4 hours of
incubation with native (282 U mL -1 ) and cutinase mutant (1524 U mL -1 ).