Surface Modification of Cellulose Acetate with Cutinase and ...

Influence of Mechanical Agitation on Cutinases and Protease Activity Toward Polyamide Substrates
reaching a high level of about 60% of adsorption. Orbital agitation provided values of
protein adsorption in the order of 30%.
K/S variation (%)
80
60
40
20
0
-20
-40
no discs with discs 4 h 24 h 48 h
vertical agitation
orbital agitation
50ºC
60ºC
70ºC
Figure 3. K/S variation, proportional to the amino groups at the surface of the treated
fabric (4% of reactive dye; liquor ratio 1:100; 90 minutes).
As obtained for polyamide model substrate (Table I), on fabric the L182A cutinase
mutant presented a considerably higher activity when compared with the native one.
(Figures 4 a) and 4 b)) Protein adsorption values are similar for both enzymes and
presented higher values when stainless steel discs were included on the enzymatic
system.
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