2012 Final Program - Pittcon

PITTCON 2012 EXHIBITOR SEMINAR LISTING
EXHIBITOR NAME ROOM # DESCRIPTION
2:00 p.m.
Introducing the New ICAP-Q ICP-MS System
Discover the dramatically different Thermo Scientific iCAP Q ICP-MS system. An all-new ultra-compact bench-top design, the iCAP Q features a
revolutionary new interface that maximizes both sensitivity and matrix tolerance, a unique ion focusing system that delivers best in class signal-to-noise
performance, the revolutionary new QCell with flatapole technology & low mass filter for unparalleled interference removal and the new QTegra software
platform that enables one-click operation, virtually eliminating the learning curve.
Tosoh Biosciences SR 04 Tuesday, March 13, 2012
10:00 a.m.
A brief overview of the analysis of biomolecules using gel filtration chromatography (GFC): common issues in method development and
troubleshooting.
Atis Chakrabarti, Ph.D., Manager, Technical Services, Laboratory Products, Tosoh Bioscience LLC
TSKgel ® size exclusion columns are known worldwide for their reliability and suitability for the analysis of proteins, peptides, and other biological
macromolecules. In 1977 Tosoh introduced TSKgel SW series high performance gel filtration columns which have become synonymous with analyzing
protein molecular weight in the field of biotechnology. TSKgel G3000XL columns are the industry standard for quality control of monoclonal antibodies.
The focus of this talk will feature an overview of TSKgel SW series gel filtration columns available from Tosoh Bioscience as well as a discussion of column
lifetime issues and common method development and troubleshooting issues encountered in size exclusion chromatography.
11:00 a.m.
Detection of Protein Heterogeneity by HPLC
Dr. Kawai, Separation Center, TOSOH Corporation
Antibodies and recombinant proteins are now widely used for therapeutic treatment, thus the evaluation of the heterogeneity of a therapeutic antibody is
essential during development, stability testing and in the quality control of the final product. In this presentation the detection and separation of protein
heterogeneity by HPLC using four different modes of chromatography is demonstrated.
High resolution nonporous resin columns for Ion Exchange Chromatography (IEX) and Hydrophobic Interaction Chromatography (HIC) were used for the
analysis of antibodies, showing a sensitivity level of 1 residue difference of the proteins. Using size exclusion chromatography (SEC) columns, baseline
separation was achieved for a monomer (150 kDa) and a dimer (300 kDa) of a human monoclonal antibody. Papain and pepsin digested antibodies were
resolved to separate Fc, Fab and intact protein to baseline resolution, also by SEC. A new reversed phase chromatography (RPC) column with a large pore
size of 300 Å is shown to be applicable for the evaluation of protein heterogeneity.
2:00 p.m.
Bridging the gap in polymer characterization: Single- and multi-detector SEC
Amandaa Brewer, Ph.D., GPC Technical Specialist, Tosoh Bioscience LLC
Since its inception, the principle use of size exclusion chromatography (SEC) has been to determine the molar mass averages and distributions of natural
and synthetic polymers. In general these properties have been characterized through the application of calibration curves via single-detector
instrumental set-ups, e.g. SEC-refractive index (RI). Over the years as the complexity of polymers has increased, the ability to obtain accurate and precise
distributions of both their physical and chemical properties have piloted a new era of polymer analysis: multi-detector SEC.
In this presentation polymer characterization via single- and multi-detector SEC, namely the coupling of SEC to various combinations of RI, UV-Vis, multiangle
light scattering (MALS), and differential viscometry (VISC), will be discussed. In addition, an overview of both multi-detector technologies as well as
applications of natural and synthetic polymers will be provided.
3:00 p.m.
Improved GPC Performance from Multiple Pore Size Chromatography Particle Technology and an Excel-based Column Selection Tool
Joe Machamer, GPC Marketing Manager, Tosoh Bioscience LLC
A novel approach to solve the linearity problems of mixed bed and multiple columns of different pore sizes linked together was developed by Tosoh
scientists and is incorporated in TSKgel SuperMultiporeHZ columns. These columns are packed with small particles of uniform size and synthesized with a
broad distribution of pore sizes. This novel approach creates a linear calibration curve within each particle. Therefore, columns with an extended linear
calibration curve can now be prepared without mixing particles of different pore sizes. Their small ID (4.6mm) and length (15cm) reduces solvent
consumption and results in quick run times, and offers high throughput capabilities.
Tosoh Bioscience has developed an Excel-based tool to plot calibration curves for various combinations of column pore size distributions. Benefits include
accurate prediction of the behavior of combinations of mixed bed columns with individual pore size columns and trade-offs between run time and
resolution.
Wednesday, March 14, 2012
10:00 a.m.
Bridging the gap in polymer characterization: Single- and multi-detector SEC
Amandaa Brewer, Ph.D., GPC Technical Specialist, Tosoh Bioscience LLC
Since its inception, the principle use of size exclusion chromatography (SEC) has been to determine the molar mass averages and distributions of natural
and synthetic polymers. In general these properties have been characterized through the application of calibration curves via single-detector
instrumental set-ups, e.g. SEC-refractive index (RI). Over the years as the complexity of polymers has increased, the ability to obtain accurate and precise
distributions of both their physical and chemical properties have piloted a new era of polymer analysis: multi-detector SEC.
In this presentation polymer characterization via single- and multi-detector SEC, namely the coupling of SEC to various combinations of RI, UV-Vis, multiangle
light scattering (MALS), and differential viscometry (VISC), will be discussed. In addition, an overview of both multi-detector technologies as well as
applications of natural and synthetic polymers will be provided.
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