AQUARICH (eco) - Kinetik

4 Studies on effectiveness/ Choice of methods/ Results
4.1 Determination of effectiveness in-vivo by using Hydrascan ®
4.1.1 Description of methods and test conditions
The test for determining the moisturizing effect of AQUARICH ® was conducted on 15 persons in an
independent testing institute. The test emulsion DAY 0402 (4% AQUARICH ® ) was tested against a
placebo and a standard moisturizer (0.1% hyaluronic acid) to determine the moisturizing effect. The
analyses were conducted on the underside of the forearm.
The skin was measured with the Hydrascan ®
application.
at 1h, 3h, 8h and 24h intervals after the last
With the specially developed Hydrascan ® method it is possible to determine the moisture in three
different skin depths. The so-called “Transient Thermal Transfer“ (TTT) enables the measurement
of skin moisture in the epidermis divided into three layers, the upper, middle and lower epidermis
layers.
The Hydrascan ® method is based on thermal conductivity and resistance which after conversion
can be used for determining skin moisture exactly.
The TTT measurement is
based on the combination of
thermal radiation/conductivity
which allows conclusions to be
drawn about the extent of skin
moisture.
The main advantage of this
measuring method is that three
different measuring zones can
be integrated in just one
measurement.
The moisturizing effect of AQUARICH ® was determined in the test during 1h, 3h, 8h and 24h on 15
persons.
One hour before the first measurement (D0) the subjects were treated as follows:
• The test persons appeared at the testing institute without pre-treatment of the arms by any
applications
• All subjects were informed about the exact procedure
• Measurement of the zero value with Hydrascan ®
• Application of a standardised amount (2μl/cm 2 ) of the test vehicle
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AQUARICH ®
(eco) │ Moisture for Skin & Hair