819 Injection Module User's Guide (PDF) - John Morris Scientific
819 Injection Module User's Guide (PDF) - John Morris Scientific
819 Injection Module User's Guide (PDF) - John Morris Scientific
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Appendix<br />
D<br />
Mobile Phase/Rinse Solvent<br />
• isocratic<br />
• 100% methanol<br />
Flow Rate<br />
0.7 mL/min<br />
<strong>Injection</strong><br />
Specifications Methods<br />
• 980 ng/mL anthracene in methanol<br />
• five times overfill total loop injection (100 µL)<br />
Rinse Volume<br />
• Probe at rinse station: aspirate 300 µL from<br />
reservoir at 15 mL/min then dispense 300 µL at<br />
16 mL/min<br />
• <strong>Injection</strong> port and valve: aspirate 450 µL from<br />
reservoir at 16 mL/min then dispense 450 µL at<br />
4 mL/min<br />
<strong>Injection</strong>s<br />
1 two system blank injections of methanol<br />
2 two initial blank injections of methanol<br />
3 one injection of 980 ng/mL anthracene<br />
4 four injections of methanol blanks<br />
Calibration Curve Parameters<br />
• external calibration<br />
• linear curve fit<br />
• five calibration levels<br />
1 0.049 ng/mL<br />
2 0.098 ng/mL<br />
3 0.294 ng/mL<br />
4 0.490 ng/mL<br />
5 0.980 ng/mL<br />
D-3
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