Manual Liquid Handling - John Morris Scientific
Manual Liquid Handling - John Morris Scientific
Manual Liquid Handling - John Morris Scientific
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MINIPULS 3<br />
Electrophysiology of rat brain cells<br />
To measure electrical signals and intracellular calcium concentrations<br />
of neuronal cells at the same time, Mr. Kwang-Hyun<br />
Cho, PhD, a postdoctoral researcher from the College of Medicine<br />
at the Catholic University of Korea, uses a microscope<br />
focusing on a perfusion chamber drained by a Minipuls 3. The<br />
neuronal cells come from a rat brain slice bathed in a buffer<br />
inside the chamber. This buffer is an oxygenated saline solution<br />
that ensures the viability of the neuronal<br />
cells, which are very sensitive to oxygen.<br />
“ ”<br />
The Minipuls 3 is very convenient<br />
for the re-circulation of solutions<br />
says Doctor Kwang-Hyun Cho when referring<br />
to a key benefit of the pump. Engineered with<br />
a 10-roller rotor, the Minipuls 3 produces<br />
smooth pulse-free flows, which eliminate<br />
siphoning or clogging phenomenon, a significant<br />
advantage when circulating sensitive<br />
neuronal cells.<br />
MINIPULS Evolution ®<br />
Study of bacterial biofilm<br />
In the Institute of Biology at the Leiden University,<br />
the molecular biology team seeks to identify Pseudomonas<br />
(Pseudomonas putida) genes and traits<br />
involved in efficient competitive root colonisation<br />
in order to understand how bacteria survive,<br />
proliferate, and compete in the rhizosphere after<br />
bacterisation of seeds. The Minipuls evolution is<br />
used to study bacterial biofilms in flowcell chambers.<br />
Bacteria grow in flowcell chambers fed by an oxygenated<br />
solution and media. The Minipuls evolution is used to<br />
bring this solution to the chambers and recycle it.<br />
The growth media was delivered at 0.125 mL/min to the flowcell<br />
system. Elen Lagendijk, technical assistant at the molecular<br />
and biology department, says<br />
“<br />
that<br />
Chamber<br />
Chamber<br />
Growth media<br />
tubing inlet<br />
Applications<br />
Inlet tubing<br />
tubing outlet<br />
Outlet tubing<br />
Flowcell chambers<br />
Minipuls 3<br />
waste<br />
bubble trap<br />
Oxygenated<br />
solution<br />
one of the outright benefits of the Gilson Minipuls evolution peristaltic<br />
pump is that it enables the operator to replace tubing, add or remove channels while the pump<br />
is running without interrupting the flow in the other chambers…<br />
”<br />
39
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