XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Lectures
NEW POSSIBILITIES FOR THE STUDY OF METABOLISM IN SLOvaKIA
Michal Kaliňák 1 and Tibor Liptaj 2
1
Department of Biochemistry and Microbiology, 2 Department of NMR and MS,
Faculty of Chemical and Food Technology Slovak University of Technology,
Radlinského 9, 812 37 Bratislava
NMR solves chemical structures for decades, but only after some laboratories in Slovakia
were equipped with new NMR spectrometers and appropriate software it is possible to
use metabonomic approach to tackle various biological questions. We have summarized
various aspects of sample preparation, measurement setup and post-acquisition processing
needed to be followed to obtain results giving biologically relevant information.
Restrictions and disadvantages of NMR in terms of sensitivity, signal overlap and sample
volume are presented.
The metabonomic 1 H NMR data of germination, growth and conidiation of filamentous
fungus Trichoderma viride are shown as an example of a study in fungal microbiology.
The onset of metabolic activity can be observed during germination. Different cultivation
conditions can be discerned on the basis of multivariate statistical analysis without the
need to identify individual metabolites.
Acknowledgement: This work was supported by Slovak Grant Agency VEGA 1/0462/08
and APVV 0642-07. NMR experimental part of this work was facilitated by the support
of Slovak National Research and Development Program No. 2003SP200280203.
66 XXII. Biochemistry Congress, Martin