XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Lectures
EvaLUATION OF TOXICITY AND GENOTOXICITY Of ORGANOHALOGEN
PESTICIDES
Pavlína Janů 1 , Markéta Thimová 1 , Petra Lovecká 1 ,
Martina Macková 1 and Kateřina Demnerová 1
Department of Biochemistry and Microbiology, Faculty of Food and Biochemical
Technology, ICT Prague, Technická 5, 166 28 Prague, Czech Republic
This work is focused on toxicity and genotoxicity analysis of the worldwide commonly
used benzonitrile herbicides dichlobenil, chloroxynil, bromoxynil and ioxynil and their
metabolites 2,6-dichlorobenzamid, 2,6-dichlorobenzoic acid, 3,5-dichloro-4-hydroxybenzoic
acid, 3,5-dibromo-4-hydroxybenzoic acid, 3,5-diiodo-4-hydroxybenzoic acid. The toxicity
was also determined for restricted organochlorine pesticides (fungicide HCB, insecticide
γ-HCH, 4,4’-DDT and its metabolites 4,4’-DDA and 4,4’-DDE).
Sea luminescent bacteria Vibrio fischeri, gramnegative bacteria Escherichia coli, grampositive
sporulating bacteria Bacillus subtilis and microorganism isolated from contaminated
soil (Burkholderia glathei) were chosen as the prokaryotic model systems for investigation
of the acute toxicity. The eukaryotic model systems were represented by the seeds
of Lactuca sativa, var. capitata and Avena sativa, by the „hairy root“ culture of Solanum
nigrum and by the human cell line HEK 293T. Genotoxicity was determinated using
the Ames test with bacteria Salmonella typhimurium His - TA98 and TA100 and the comet
assay with the HEK 293T cell line.
Our data point to that toxicity of pesticides is higher than their corresponding metabolites
practically in all used systems. The toxic effect of studied compounds was similar in the
context of used model system. The comet assay confirmed genotoxicity of pesticides
ioxynil, bromoxynil, hexachlorobenzene and DDE, the metabolite of DDT.
Acknowledgements: The authors thank for the support of grants Tandem FT –TA4/101,
FT-TA5/043 a MSM 6046137305.
XXII. Biochemistry Congress, Martin
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