XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Lectures
PROTEIN IMMUNIZATION OF MUTANT MOUSE – AN EffICIENT WAY
TO GENEraTE SELECTIVE AND SENSITIVE ANTIBODIES
Anna Hrabovska 1,2 , Veronique Bernard 1 and Eric Krejci 1
1
Centre d’Etude de la Sensori-Motricité, Université Paris Descartes- CNRS- UMR8194,
45 rue des Saints Pères, 75006 Paris, France
2
Dpt. of pharmacology and toxicology, Faculty of Pharmacy, Comenius University,
Odbojarov 10, 832 32 Bratislava
Despite a long history, the successful generation of specific and selective antibodies is
still a task fraught with considerable uncertainty.
We examine a simple, fast, and highly efficient strategy to produce an antibody, which
utilizes immunization of mutant mouse strains with antigens that the host strains themselves
have been genetically targeted to be deficient for. To test this strategy we choose
butyrylcholinesterase, an antigen that has been considered to be difficult to generate
antibody against. Antigens of different origins all provided a strong immune response,
while the characteristic of the resulting antibodies depended on the preparation for the
antigen prior to the immunization.
This method, introduced previously but since neglected until now due probably to the
lack of specific resources, should at this time, based on our data presented here, be
considered a reasonable and reliable choice for antibody production.
Acknowledgement: Work was supported by grants AFM; grant ANR Neuroscience and
APVV grants (SK-FR-0031-09 a SK-CZ-0028-09).
XXII. Biochemistry Congress, Martin
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