XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
118.
QUALITY AND TIME-STABILITY OF HUMAN SKIN EXPLANTS
Alena Rajnochová Svobodová 1 , Adéla Zdařilová 1 , Dana Kylarová 2 ,
Bohumil Zálešák 3 and Jitka Vostálová 1
1
Department of Medical Chemistry and Biochemistry, Palacký University Olomouc,
2
Department of Histology and Embryology, Palacký University Olomouc,
3
Department of Plastic and Aesthetic Surgery, Faculty Hospital, Olomouc
Human skin obtained from plastic surgery has been used for isolation of skin cells such
as keratinocytes, fibroblasts, melanocytes or endothelial cells for several decades. Single
cell cultures, co-cultures or 3D cultures of these cells have been utilized for dermatological
studies including phototoxicity and photoprotection investigation. However, these
very simple systems are very different from human skin. Therefore cultivation of human
skin organ culture (ex vivo skin) has been developed as a more suitable model system.
The aim of this study was to determine explant usage period (quality and stability
during cultivation). Morphological structure and antioxidant parameters (activity of
superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione S-transferase
(GST) and catalase (CAT) and level of thiobarbituric acid-reactive substances (TBARS)
and glutathione (GSH) were evaluated. Epidermis did not show any significant changes
in its morphology until 72 h cultivation. In dermis moderate acute inflammatory reaction
was observed (48 - 72 h) but it disappeared after 120 h. Dermal papillae started to be
reduced after 96 h and these changes became more significant after 120 h cultivation.
TBARS level together with GSH amount and activity of SOD have markedly increased
for the cultivation period (0 - 144 h). Activities of GPX and CAT have grown for 72 - 96 h
and then decreased. Changes in GST activity did not have any trend and varied between
70 - 160 % of control (0 h).
In conclusion human skin explants can be utilized for various dermatological applications
including photoprotection or metabolic and xenobiochemic studies.
Acknowledgement: This work was financially supported by the grant MSM 6198959216.
XXII. Biochemistry Congress, Martin
243