XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
109.
THE EffECT OF BENDIOCarB ON ANTIOXIDANT ParaMETERS IN MALE
AND FEMALE ORGANS OF raBBIT
Anna Sobeková 1 , Ľuboslava Lohajová 1 and Peter Javorský 2
1
Department of Chemistry, Biochemistry and Biophysics UVLF in Košice
2
Institute of Animal Physiology SAV Košice
The toxicity of bendiocarb is believed to be connected with the acetylcholine esterase
inhibition. However, the toxic effect of pesticides is cumulated from all their metabolites.
Male and female rabbits were orally administered bendiocarb at a dose 5 mg.kg -1 body
weight for 30 days. Administration of bendiocarb resulted in a significant alterations of
antioxidant and detoxifying enzymes activities (SOD-superoxide dismutase, CAT-catalase,
GPx-glutathione peroxidase) in liver and kidney of male and female rabbits. In the male
liver, the activities of SOD and CAT were not affected by bendiocarb, but changes in SOD
isoenzymes pattern were observed in the experimental groups. The activities of GPx
were significantly decreased on the 3rd and the 10th day of an experiment. Significantly
higher levels of TBARS indicate the oxidative stress of the organ. The activity of GPx does
not change in female liver. In the kidney, the activities of all followed enzymes changed
without significant increasing of TBARS.
The inhibition of antioxidant defence system, increased TBARS values and changes in
SOD isoenzyme pattern showed that the toxic effect of bendiocarb is not only in the
acetylcholine esterase inhibition, but probably also in ROS production. ROS can be produced
by cyclic biotransformation of bendiocarb and its metabolites. Our results also
show the alterations in the level of some antioxidant parameters regarding variation in
the response of male and female animals.
Acknowledgement: This work was supported by grant project VEGA 2/0021/09.
234 XXII. Biochemistry Congress, Martin