XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta
XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta
XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta
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Posters<br />
107.<br />
ActivITIES of drUG-METaBOLIZING aND aNTIOXIDaNT ENZYMES<br />
IN Haemonchus contortus STraINS rESISTaNT or SENSITIve TO<br />
aNTHELMINTICS<br />
Tamara Lasotová 1 , Hana Bártíková 1 , Ivan Vokřál 1 , Barbora Szotáková 1 ,<br />
Vladimír Kubíček 1 , Jiří Lamka 1 , Marián Várady 2 and Lenka Skálová 1<br />
1<br />
Faculty of Pharmacy, Charles University, Hradec Králové, Czech Republic<br />
2<br />
Institute of Parasitology, Slovak Academy of Sciences, Košice, Slovakia<br />
Haemonchus contortus is one of the most pathogenic parasites of small ruminants (e.g.,<br />
sheep and goat). The treatment of haemonchosis is complicated because of frequent<br />
resistance of H. contortus to common anthelmintics. The aim of this project was to<br />
compare the activities of antioxidant enzymes and biotransformation enzymes toward<br />
selected anthelmintics and model xenobiotics in three different strains of H. contortus:<br />
ISE strain (sensitive to common anthelmintics), BR strain (resistant to benzimidazole<br />
anthelmintics) and WR strain (resistant to all common anthelmintics). For this purpose,<br />
lambs were experimentally infected with H. contortus L3 larvae and then adults<br />
worms were collected from ovine abomasums. The biotransformation of anthelmintics<br />
albnedazole and flubendazole was tested in vitro (in subcellular fractions of H. contortus<br />
homogenate) as well as ex vivo (in living nematodes cultivated in flasks with medium).<br />
In vitro, the activities of several carbonyl-reducing enzymes, UDP-glucosyltransferase,<br />
glutathione-S-transferases, peroxidases, catalase, and superoxid dismutase toward model<br />
substrates were tested. The acquired data showed significant differences in tested activities<br />
between sensitive and resistant H. contortus strains. The altered activities of certain<br />
detoxifying enzymes could protect some parasites against the toxic effect of the drugs<br />
and contribute to drug-resistance of these parasites.<br />
Acknowledgement: This project was supported by the Czech Science Foundation, grant<br />
No. P502/10/0217.<br />
232 <strong>XXII</strong>. Biochemistry Congress, Martin
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