XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
105.
SELENITE-INDUCED CELL DEATH IN HUMAN COLON CANCER CELLS
Věra Králová and Emil Rudolf
Department of Medical Biology and Genetics, Faculty of Medicine in Hradec Králové
Selenium compounds have been shown to play role in chemoprevention of tumours.
Sodium selenite was reported to inhibit proliferation and induce different types of cell
death in cancer cells in vitro. In our study we tested the effects of sodium selenite on
cell proliferation and cell death in human colon cancer cell line HCT 116. Cell proliferation
was measured by xCELLigence impedance method. Cell morfology was followed by
time-lapse videomicroscopy. Caspase activity and mitochondrial membrane potential
were determined by flow cytometry. Activation of DNA damage response was detected
using western blotting and immunofluorescence. Sodium selenite at concentration of 10
µM induced cell death in HCT 116 cells characterised by cell detachment and rounding,
decrease in mitochondrial membrane potential and activation of caspases. Seleniteinduced
cell death was prevented with antioxidant MnTMPyP and enhanced by depletion
of reduced glutathion with BSO, which suggests role of oxidative stress. The process was
accompanied by increase in ATM kinase and histone H2A.X phosphorylation.
Acknowledgements: This work was supported by Charles University Grant Agency (GAUK)
Research Project 129609.
230 XXII. Biochemistry Congress, Martin