XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
98.
EffECT OF N-3 POLYUNSATUraTED faTTY ACIDS SUPPLEMENTATION ON
raT LIVER IN DIffERENT THYROID STATUSES
Martina Vokurková 1,2 , Hana Rauchová1,2 , Stanislav Pavelka 1 ,
Tomáš Soukup 1 and Narcis Tribulová3
1
Institute of Physiology, Academy of Science of the Czech Republic, 2 Centre for
Cardiovascular Research, Prague, Czech Republic, 3 Institute for Heart Research,
Slovak Academy of Sciences, Bratislava, Slovak Republic
It is known that thyroid hormones influence lipid metabolism and oxidative stress.
Epidemiological studies demonstrate that n-3 polyunsaturated fatty acids (PUFAs) consumption
is associated with a reduced risk of atherosclerosis and hyperlipidemia. The aim
of our study was to test how PUFAs supplementation in different thyroid statuses of rat
can affect lipid metabolism and parameters of oxidative stress in the liver. The different
thyroid statuses of the experimental groups (euthyroid, hyperthyroid and hypothyroid)
were defined by the levels of the thyroid hormones in the plasma and an activity of liver
mitochondrial glycerol-3-phosphate dehydrogenase. In this study, the hyperthyroid rats
had significantly decreased levels of total plasma cholesterol and LDL-cholesterol than
the hypothyroid rats. The levels of HDL-cholesterol were increased in the hyperthyroid
rats in comparison with the euthyroid rats. The concentrations of liver thiol groups differed
according to the thyroid statuses (they were the highest in the hypothyroid group
and the lowest in the hyperthyroid group). However, no differences in the content of
conjugated dienes were observed among the experimental groups. PUFAs supplementation
thus, in our experimental design, modified neither lipid metabolism nor parameters
of the oxidative stress in the liver.
Acknowledgements: This work was supported by the GACR (303/09/0570) and Ministry
of Education, Youth and Sport (1M6798582302 and AV0Z 50110509).
222 XXII. Biochemistry Congress, Martin