XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
90.
effECT of OMEGa-3 faTTY aCIDS ON PON 1 arYLESTEraSE
aND laCTONaSE aCTIvITY IN CHILDrEN SUfferING frOM
HYPErCHOLESTErOLEMIa
Lucia Andrezálová, Zuzana Országhová, Jana Muchová and Zdeňka Ďuračková
Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry,
Faculty of Medicine, Comenius University Bratislava, Slovakia
Hypercholesterolemia is characterized by an increased level of blood cholesterol and give
rise to the development of atherosclerosis. The atherosclerosis process begins already in
childhood and is the main risk factor that contributes to the pathology of cardiovascular
diseases in adulthood.
Paraoxonase 1 (Pon 1), HDL-associated enzyme with antioxidative properties, plays an
important role in the antiatherogenic processes. The aim of this study was to determine
PON 1 arylesterase and lactonase activity in 35 moderately hypercholesterolemic children
(average age 16 ± 3.34) and study the changes in its activity during the daily administration
of nutritional supplement (CW) comprising of omega-3 fatty acids (1000 mg EPA/
DHA) and phytosterol esters (1300 mg) over a 16 week period PON 1 arylesterase and
lactonase activities were determined spectophotometrically towards phenylacetate and
dihydrocoumarin in serum samples. CW supplementation did not significantly affect PON 1
activities. While arylesterase activity was within the physiological range, lactonase activity
was moderately reduced compared to healthy subjects. PON 1 activities were correlated
with other biochemical parameters as well as with lipid peroxides and homocysteine.
Acknowledgement: Authors wish to thank for partial financial support to Obsidian Research
Limited, Pork Talbot (Great Britain), Mind and Health, civil association and MVTS and
VEGA grants of Ministry of Education SR.
214 XXII. Biochemistry Congress, Martin