XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
89.
THE LysM DOMaIN IN SUrfaCE IMMUNOGENIC prOTEIN (Sip) aND ITS
INfLUECE ON ELICITaTION of IMMUNITY agaINST StrePTocoCCus
agalaCTIae
Barbora Vidová, Michal Chotár and Andrej Godány
Institute of Molecular Biology, SAS Bratislava
The microbial pathogens are characterized by adapting their surface proteins in order to
protect themselves against the host immune system; accordingly, there is a possibility
of some variability also within the sip gene. This work was focused on determining of
potential variability within the sip gene in the collection of bovine isolates of S. agalactiae
by multiplex PCR method and characterizing the protein Sip on its nucleotide level,
with the aim to study its probable variability and prepare the basis for future study
of its epitope responsible for immunity elicitation. Concurrently, we also examined
the presence of a peptidoglycan-binding anchor, the LysM domain motif. Because the
identification of both variability and anchor motif is important for the identification of
a potential epitope within the Sip, the immune responses to whole and partial Sip were
also tested in the mice model.
Acknowledgements: This work was supported by the VEGA grant no. 2/0121 of the Slovak
Academy of Sciences.
212 XXII. Biochemistry Congress, Martin