XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
81.
ApplicaTION of CONCENTraTION fLUOrESCENCE matrICES TO THE
DETECTION of fLUOrESCENCE METaBOLITES IN urINE
Lucia Lichardusová, Jaroslav Kušnír and Mária Mareková
Institute of Chemistry, Biochemistry, Medical Biochemistry and LABMED a.s.,
UPJŠ Košice
Urine contains a variety of organic and inorganic chemicals, including a number of
natural fluorophores, most of which are tryptophan metabolites. The alteration in the
autofluorescence of urine could result from both physiological and pathological changes.
The synchronous fluorescence spectrum (SFS) is considered to be the characteristic “fingerprint“,
because it is unique for a given mixture and it characterizes it graphically as one
unit. By introducing concentration parameter, the SFS form concentration fluorescence
matrices which provide the information of the precise mixture ratios of the urine samples.
In this work we present application of concentration fluorescence matrices to the detection
of urine fluorophores, particularly tryptophan and tyrosine metabolites. The SFS
include autofluorescence of individual fluorophores and their interaction. Every alternation
of one fluorophor disturbs balance of fluorophores interaction and this is shown by
alternation graphic record. The CSMF spectra were formed by the SFS at various Δλ. As
the urine is a complex multi-component system, the shape and intensity of the spectra
obtained under different Δλ changed distinctly and contained rich and varied information.
Acknowledgement: This study was supported by VEGA 1/0402/10.
204 XXII. Biochemistry Congress, Martin